Optimization and standardization of mNGS-based procedures for the diagnosis of Mycoplasma periprosthetic joint infection: A novel diagnostic strategy for rare bacterial periprosthetic joint infection

Yuanqing Cai; Haiqi Ding; Xiaoqing Chen; Yang Chen; Changyu Huang; Chaofan Zhang; Zida Huang; Ying Huang; Wenbo Li; Wenming Zhang; Xinyu Fang

doi:10.3389/fcimb.2023.1089919

Optimization and standardization of mNGS-based procedures for the diagnosis of Mycoplasma periprosthetic joint infection: A novel diagnostic strategy for rare bacterial periprosthetic joint infection

Front Cell Infect Microbiol. 2023 Mar 1:13:1089919. doi: 10.3389/fcimb.2023.1089919. eCollection 2023.

Authors

Yuanqing Cai^{1

2

3}, Haiqi Ding^{1

3}, Xiaoqing Chen⁴, Yang Chen^{1

3}, Changyu Huang^{1

3}, Chaofan Zhang^{1

3}, Zida Huang^{1

3}, Ying Huang^{1

3}, Wenbo Li^{1

3}, Wenming Zhang^{1

3}, Xinyu Fang^{1

3}

Affiliations

¹ Department of Orthopedics, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, Fuzhou, Fujian, China.
² Department of Orthopedics, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
³ Department of Orthopedics, The First Affiliated Hospital, Fujian Medical University, Fuzhou, Fujian, China.
⁴ Department of Orthopedics, Quanzhou First Hospital Affiliated to Fujian Medical University, Quanzhou, China.

Abstract

Introduction: The diagnosis of Mycoplasma periprosthetic joint infection (PJI) is rather difficult due to its rarity and difficult in isolation, there are not standardized diagnostic procedure for Mycoplasma PJI presently. This study aimed to reported a metagenomic next-generation sequencing (mNGS)-based diagnostic strategy for Mycoplasma PJI.

Methods: In the present study, we have reported the largest number of Mycoplasma PJI that were precisely diagnosed by mNGS and verified by optimized microbial culture methods and (or) 16S PCR polymerase chain reaction (PCR).

Results: The positive rate of optimized microbial culture methods and 16S PCR in the detection of Mycoplasma PJI was 57.14% and 71.43%, respectively. The infections were well controlled by targeted treatment in all cases.

Conclusion: The standardized and optimized procedure based on mNGS presented in this study is useful for the diagnosis of Mycoplasma PJI, which might also be provided as a novel diagnostic strategy for rare bacterial PJI.

Keywords: Mycoplasma; metagenomic next-generation sequencing; periprosthetic joint infection; procedure; rare bacteria.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arthritis, Infectious* / microbiology
Bacteria
Bacterial Infections*
Humans
Mycoplasma Infections* / diagnosis
Mycoplasma* / genetics
Prosthesis-Related Infections* / diagnosis
Prosthesis-Related Infections* / microbiology
Reference Standards
Sensitivity and Specificity

Grants and funding

This work was supported by the National Natural Science Foundation of China (82072458, 82171370, 82102621, 82272510), Foreign Cooperation Project of Science and Technology, Fujian Province (No.2021I0012), 2019-2020 Middle-aged and Young Experts with Outstanding Contributions to Health in Fujian Province, Fujian Orthopaedic Bone and Joint Disease and Sports Rehabilitation Clinical Medical Research Center (2020Y2002), Natural Science Foundation of Fujian Province (2022J011457, 2022J011357), Quanzhou Science and Technology Plan Project (2021N061S), Joint Funds for the Innovation of Science and Technology, Fujian province (2019Y9136), Fujian Provincial Health Technology Project (2022GG007).