An efficient transformation method for tannin-containing sorghum

Yuan Liang; Xuehui Yan; Jingyi Xu; Yanlong Liu; Ke Xie; Jieqin Li; Qiuwen Zhan

doi:10.7717/peerj.15066

An efficient transformation method for tannin-containing sorghum

PeerJ. 2023 Mar 14:11:e15066. doi: 10.7717/peerj.15066. eCollection 2023.

Authors

Yuan Liang¹, Xuehui Yan¹, Jingyi Xu¹, Yanlong Liu¹, Ke Xie², Jieqin Li¹, Qiuwen Zhan¹

Affiliations

¹ College of Agricultural, Anhui Science and Technology University, Fengyang, Anhui, China.
² Research Center of Biology and Agriculture, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, China.

Abstract

Background: Tannins are the main bottlenecks restricting the transformation efficiency of plants. Hongyingzi is a special tannin-containing sorghum cultivar used in brewing.

Methods: In this study, a highly efficient microprojectile transformation system for tannin-containing sorghum was successfully exploited using immature embryos (IEs) of Hongyingzi as explants.

Results: Hongyingzi presented two types of calli. Type II calli were found to be the most suitable and effective explants for transformation. After optimization of the geneticin (G418) concentration and tissue culture medium, an average transformation frequency of 27% was achieved. Molecular analyzis showed that all transgenic plants were positive and showed transgenes expression. The inheritance analyzis confirmed that the transgenes could be inherited into the next generation. Thus, we successfully established an efficient transformation system for tannin-containing sorghum and demonstrated the possibility of breaking the restriction imposed by tannins in plants.

Keywords: Genetic transformation; Particle bombardment; Tannin-containing sorghum; Tissue culture; Transgenic plants.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Edible Grain
Plants, Genetically Modified / genetics
Sorghum* / genetics
Tannins* / metabolism
Transformation, Genetic
Transgenes

Substances

Tannins

Grants and funding

This work was supported by the National Key R&D Program of China (2018YFD1000702/2018YFD1000700), the National Natural Science Foundation of China (31971993), the Key-construction Subject Plan of Anhui Province (WanJiaoMiKe[2014]28), the National Key Research and Development Program of China (2018YFD0300902), the Anhui Provincial Natural Science Fund (2008085MC73), and the Key Project of Natural Science Research of Anhui Provincial Education Department (KJ2021ZD0108). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.