Tailoring the whole-cell sensing spectrum with cyborgian redox machinery

Kai Yang; Zi-Jie Lu; Tian-Yu Zhu; Jing-Xian Wang; Fu-Qiao Yang; Syed Zaghum Abbas; Jun Zhou; Zhugen Yang; Jian-Li Mi; Sai Kishore Ravi; Yang-Chun Yong

doi:10.1016/j.aca.2023.341046

Tailoring the whole-cell sensing spectrum with cyborgian redox machinery

Anal Chim Acta. 2023 Apr 29:1252:341046. doi: 10.1016/j.aca.2023.341046. Epub 2023 Mar 3.

Authors

Affiliations

¹ Biofuels Institute, Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, School of Emergency Management & School of Environment and Safety Engineering, Zhenjiang, 212013, Jiangsu Province, China.
² Bioenergy Research Institute, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816, Jiangsu Province, China.
³ School of Water, Energy and Environment, Cranfield University, Milton Keynes, MK43 0AL, United Kingdom.
⁴ Institute for Advanced Materials, School of Materials Science and Engineering, Jiangsu University, Zhenjiang, 212013, Jiangsu Province, China.
⁵ School of Energy and Environment, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong Special Administrative Region of China.
⁶ Biofuels Institute, Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, School of Emergency Management & School of Environment and Safety Engineering, Zhenjiang, 212013, Jiangsu Province, China. Electronic address: ycyong@ujs.edu.cn.

PMID: 36935150
DOI: 10.1016/j.aca.2023.341046

Abstract

Whole-cell biosensors are an important class of analytical tools that offer the advantages of low cost, facile operation, and unique reproduction/regeneration ability. However, it has always been quite challenging to expand the sensing spectrum of the host. Here, a new approach to extend the cell sensing spectrum with biomineralized nanoparticles is developed. The nano-biohybrid design comprise biomineralized FeS nanoparticles firmly anchored onto the bacterium, Shewanella oneidensis MR-1, wherein the nanoparticles are wired to the cellular electron transfer machinery (MtrCAB/OmcA) of the bacterium, forming an artificial cyborgian redox machinery consisting of FeS-MtrCAB/OmcA-FccA. Strikingly, with this cyborgian redox machinery, the sensing spectrum of FeS hybridized S. oneidensis MR-1 cell is successfully expanded to enable whole-cell electrochemical detection of Vitamin B12, while an unhybridized native cell is incapable of sensing. This proof-of-concept nano-biohybrid design offers a new perspective on manipulating the microbial toolkit for an expanded sensing spectrum in whole-cell biosensors.

Keywords: Bioelectrochemistry; Biosensor; Shewanella oneidensis; Vitamin B12.

MeSH terms

Electron Transport
Nanoparticles*
Oxidation-Reduction
Shewanella*