The design of a point of care FET biosensor to detect and screen COVID-19

Abstract

Graphene field effect transistor (FET) biosensors have attracted huge attention in the point-of-care and accurate detection. With the recent spread of the new emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the need for rapid, and accurate detection & screening tools is arising. Employing these easy-to-handle sensors can offer cheap, rapid, and accurate detection. Herein, we propose the design of a reduced graphene oxide (rGO) FET biosensor for the detection of SARS-CoV-2. The main objective of this work is to detect the SARS-CoV-2 spike protein antigen on spot selectively and rapidly. The sensor consists of rGO channel, a pair of golden electrodes, and a gate underneath the channel. The channel is functionalized with COVID-19 spike protein antibodies to achieve selectivity, and with metal nanoparticles (MNPs) such as copper and silver to enhance the bio-sensing performance. The designed sensor successfully detects the SARS-CoV-2 spike protein and shows singular electrical behavior for detection. The semi-empirical modeling approach combined with none-equilibrium Green's function were used to study the electronic transport properties of the rGO-FET biosensor before and after the addition of the target molecules. The sensor's selectivity is also tested against other viruses. This study provides a promising guide for future practical fabrication.