Glucose oxidase-loaded colloidal stable WS2 nanobowls for combined starvation/photothermal therapy of colorectal tumors

Zheng Chen; Xiaoyi Zheng; Jiulong Zhao; Jingwen Tang; Lianghao Hu; Shige Wang

doi:10.1016/j.ijpharm.2023.122848

Glucose oxidase-loaded colloidal stable WS₂ nanobowls for combined starvation/photothermal therapy of colorectal tumors

Int J Pharm. 2023 Apr 5:636:122848. doi: 10.1016/j.ijpharm.2023.122848. Epub 2023 Mar 15.

Authors

Zheng Chen¹, Xiaoyi Zheng², Jiulong Zhao², Jingwen Tang¹, Lianghao Hu², Shige Wang³

Affiliations

¹ School of Materials and Chemistry, the University of Shanghai for Science and Technology, No. 516 Jungong Road, Shanghai 200093, PR China.
² Department of Gastroenterology, Changhai Hospital, Naval Military Medical University, No. 168 Changhai Road, Shanghai 200433, PR China.
³ School of Materials and Chemistry, the University of Shanghai for Science and Technology, No. 516 Jungong Road, Shanghai 200093, PR China. Electronic address: sgwang@usst.edu.cn.

PMID: 36931536
DOI: 10.1016/j.ijpharm.2023.122848

Abstract

Glucose is used as an important nutrient to support cell growth. The glucose oxidase (GOx) can transform glucose into gluconic acid and toxic H₂O₂, which can be used for tumor starvation therapy. However, the leakage of GOx may cause severe side effects to the normal tissue. To prevent the accidental leakage of GOx, this study proposes the chemical modification of GOx on the photothermal transducing agent surface, to realize the safe and combined starvation and photothermal therapy of colorectal tumors. Polyvinylpyrrolidone (PVP)-modified WS₂ nanobowls (WS₂-PVP) as a photothermal transducing agent were produced using a one-pot preparation method. Then, α-lipoic acid (LA) molecules were immobilized at the sulfur-deficient sites on the surface of WS₂ nanobowls to afford the chemical loading of GOx through amide bonds. Under the irradiation of a near-infrared laser (808 nm), thermal energy is generated by WS₂ to kill colorectal cancer cells locally. The photothermal conversion efficiency of WS₂-PVP-LA was 27.2%. This study is anticipated to open up an alternative avenue for the rational design of multifunctional nanotherapeutics for tumor therapy.

Keywords: Colorectal tumor; Glucose oxidase; Photothermal therapy; WS(2) nanobowls.

MeSH terms

Cell Line, Tumor
Colorectal Neoplasms* / drug therapy
Glucose
Glucose Oxidase / chemistry
Humans
Hydrogen Peroxide / chemistry
Nanoparticles* / chemistry
Neoplasms* / drug therapy
Photothermal Therapy
Povidone

Substances

Glucose Oxidase
Hydrogen Peroxide
Povidone
Glucose