Di-n-butyl phthalate stress hampers compost multifunctionality by reducing microbial biomass, diversity and network complexity

Abstract

Phthalates are common pollutants in agriculture. Here, the influence of di-n-butyl phthalate (DBP) on multifunctionality of composting was assessed. Results indicated that DBP stress (100 mg/kg) hampered multifunctionality from the thermophilic phase onwards and resulted in a 6.5 % reduction of all assessed functions. DBP stress also significantly reduced microbial biomass (P < 0.05), altered microbial composition (P < 0.05), and decreased network complexity (P < 0.01). Multifunctionality was found to be strongly correlated (P < 0.001) with microbial biomass, diversity, and network complexity. In addition, keystone taxa responsive to DBP were identified as Streptomyces, Thermoactinomyces, Mycothermus, and Lutispora. These taxa were significantly (P < 0.001) affected by DBP stress, and a correlation between them and multifunctionality was shown. This study contributes to a better understanding of the negative implications of phthalates during composting processes, which is of great significance to the development of new treatment strategies for agricultural waste.

Keywords: Composting; Di-n-butyl phthalate; Microbial diversity; Multifunctionality; Network complexity.