Serum carboxymethyllysine concentration is associated with erosive hand osteoarthritis

A Cambon-Binder; S Jaisson; S Tuffet; A Courties; F Eymard; A Okwieka; P Gillery; A Miquel; A Rousseau; M D Crema; F Berenbaum; J Sellam

doi:10.1016/j.joca.2023.03.006

Serum carboxymethyllysine concentration is associated with erosive hand osteoarthritis

Osteoarthritis Cartilage. 2023 Jul;31(7):976-984. doi: 10.1016/j.joca.2023.03.006. Epub 2023 Mar 15.

Authors

A Cambon-Binder¹, S Jaisson², S Tuffet³, A Courties⁴, F Eymard⁵, A Okwieka², P Gillery², A Miquel⁶, A Rousseau³, M D Crema⁷, F Berenbaum⁴, J Sellam⁸

Affiliations

¹ Sorbonne University, Assistance Publique-Hôpitaux de Paris (AP-HP), Orthopaedic and Upper Limb Surgery Department, Saint-Antoine Hospital, Paris, France; Centre de Recherche Saint-Antoine, INSERM UMRS_938, Paris, France.
² MEDyC Unit CNRS UMR n° 7369, Faculty of Medicine, University of Reims Champagne-Ardenne, Reims, France; Biochemistry Department, University Hospital of Reims, Reims, France.
³ Sorbonne University, AP-HP, Service de Pharmacologie Clinique et Plateforme de Recherche Clinique de l'Est Parisien (URCEST, CRB, CRC), Saint-Antoine Hospital, Paris, France.
⁴ Centre de Recherche Saint-Antoine, INSERM UMRS_938, Paris, France; Sorbonne University, AP-HP, Rheumatology department, Saint-Antoine Hospital, Paris, France.
⁵ Department of Rheumatology, AP-HP, Henri Mondor Hospital, 94000 Créteil, France.
⁶ Sorbonne University, AP-HP, Radiology Department, Saint-Antoine Hospital, Paris, France.
⁷ Institut d'Imagerie du Sport, Institut National du Sport, de l'Expertise et de la Performance (INSEP), Paris, France.
⁸ Centre de Recherche Saint-Antoine, INSERM UMRS_938, Paris, France; Sorbonne University, AP-HP, Rheumatology department, Saint-Antoine Hospital, Paris, France. Electronic address: jeremie.sellam@aphp.fr.

PMID: 36931384
DOI: 10.1016/j.joca.2023.03.006

Abstract

Objective: Carboxymethyllysine (CML) and homocitrulline (HCit) are the products of two non-enzymatic post-translational modifications of protein, a process related to age. We investigated whether serum CML and HCit concentrations were associated with hand osteoarthritis (HOA), especially erosive HOA.

Design: Serum CML and HCit were measured by using liquid chromatography coupled with tandem mass spectrometry at inclusion in 386 patients included in the DIGItal Cohort Design (DIGICOD) cohort. We investigated whether serum CML and/or HCit concentrations were associated with erosive HOA or with HOA clinical and radiological features. Moreover, we compared the tissular concentrations of CML and HCit in OA and non-OA cartilage from proximal interphalangeal and metacarpo-phalangeal (MCP) joints from human cadaveric donors.

Results: Median (IQR) serum CML concentration was lower in patients with erosive HOA than those with non-erosive HOA (178.7 [157.1-208.8] vs 194.7 [168.9-217.1] μmol/mol Lys, P = 0.002), but median HCit concentration did not differ between the groups (193.9 [162.9-232.0] vs 193.9 [155.9-224.6] μmol/mol Lys). Cartilage HCit and CML concentrations were not correlated with clinical features. Serum CML concentration was higher in OA than non-OA MCPs (7.0 vs 4.0 mmol/mol Lys, P = 0.01).

Conclusions: Serum CML concentration was lower in erosive HOA than non-erosive HOA, and cartilage CML concentration was higher in OA than non-OA cartilage. These results encourage further studies to test whether serum CML could be a new prognostic biomarker in HOA.

Keywords: Advanced glycation end-products; Biomarker; Carbamylation; Carboxymethyllysine; Erosive hand osteoarthritis; Homocitrulline.

MeSH terms

Hand
Hand Joints* / diagnostic imaging
Humans
Osteoarthritis* / diagnostic imaging
Radiography

Substances

N(6)-carboxymethyllysine