Salinibacterium sedimenticola sp. nov., Isolated from Tidal Flat Sediment

Abstract

An actinobacterium, designated as SYSU T00001^T, was isolated from a tidal flat sediment sample from Guangdong province, China. Cells were Gram-stain-positive, aerobic, motile and short rod-shaped. Colonies on marine agar 2216 were smooth, yellow-pigmented, and circular with low convexity. The isolate was able to grow at the temperature range 4-37 °C (optimum 30 °C), at pH 4.0-10.0 (optimum 7.0) and in the presence of 0-10% (w/v) NaCl. The major menaquinones were MK-11 and MK-10. The cell wall contained alanine, glutamic acid, lysine and ornithine. The major fatty acids were C_19:0 cyclo ω8c (35.7%) and anteiso C_15:0 (26.0%). The polar lipids consisted of one diphosphatidyl glycerol, one unidentified glycolipid and one unknown lipid. Whole genome sequencing of strain SYSU T00001^T revealed 2,837,702 bp with a DNA G + C content of 67.8%. Phylogenetic analyses clearly demonstrated that strain SYSU T00001^T belonged to the genus Salinibacterium, and the highest 16S rRNA gene similarity to Salinibacterium hongtaonis 194^T (97.8%). The ANI and dDDH values of strain SYSU T00001^T relative to Salinibacterium hongtaonis 194^T were 74.5% and 19.5%, respectively. According to our data, strain SYSU T00001^T represents a novel species of the genus Salinibacterium, for which the name Salinibacterium sedimenticola sp. nov. is proposed, the type strain is SYSU T00001^T (= GDMCC 1.3283^T = KCTC 49758^T).