The complete mitochondrial genome of a wild-collected Kappaphycus malesianus (Solieriaceae, Rhodophyta)

Bea A Crisostomo; Richard V Dumilag; Michael Y Roleda; Arturo O Lluisma

doi:10.1080/23802359.2023.2183728

The complete mitochondrial genome of a wild-collected Kappaphycus malesianus (Solieriaceae, Rhodophyta)

Mitochondrial DNA B Resour. 2023 Mar 10;8(3):359-363. doi: 10.1080/23802359.2023.2183728. eCollection 2023.

Authors

Bea A Crisostomo¹, Richard V Dumilag^{2

3}, Michael Y Roleda¹, Arturo O Lluisma¹

Affiliations

¹ The Marine Science Institute, College of Science, University of the Philippines, Diliman, Quezon City, Philippines.
² Fisheries Department, Sorsogon State University, Magallanes Campus, Aguada Norte, Magallanes, Sorsogon, Philippines.
³ Institute of Oceanography and Environmental Science, Mindanao State University, Tawi-Tawi College of Technology and Oceanography, Boheh Sallang, Sanga-Sanga, Bongao, Tawi-Tawi, Philippines.

Abstract

Kappaphycus malesianus is a red seaweed farmed primarily for its carrageenan, a polysaccharide important in the food and pharmaceutical industries. Among the commercially cultivated Kappaphycus species, only K. malesianus has no mitogenome data available. Here, we assembled the mitochondrial genome of K. malesianus from next-generation sequencing data. The circular mitogenome consisted of 25,250 base pairs (bp) with a GC content of 30.25%. These values were comparable to previously sequenced solieriacean mitogenomes. Structural features, such as the stem-loop and hairpin, which were previously reported in other rhodophytes mitochondrial DNA, were also identified. The annotated genes (24 protein-coding genes, 24 tRNA genes, and 2 rRNA genes) were arranged in an order similar to the other available solieriacean mitogenomes. Lastly, phylogenetic analysis using 23 predicted protein domains showed the sister relationship of K. malesianus with other Kappaphycus species.

Keywords: Rhodophyta; eucheumatoids; mitochondrial genome; phylogenetics.

Grants and funding

This work was funded by the United Kingdom Research and Innovation–Global Challenges Research Fund (UKRI-GCRF) ‘GlobalSeaweedSTAR’ Programme GSS/RF/015 [BB/P027806/1] and by the Commission on Higher Education under the Leading the Advancement of Knowledge in Agriculture and Science (CHED – LAKAS) Project, Phytochemical characterization of macroalgae for food and high-value products (PhycoPRO) (LAKAS 2021-035) awarded to MYR and RVD.