Optimizing the biodegradability and osteogenesis of biogenic collagen membrane via fluoride-modified polymer-induced liquid precursor process

Xiyan Li; Chuangji Li; Mengxi Su; Xinyi Zhong; Yihan Xing; Zhengjie Shan; Shoucheng Chen; Xingchen Liu; Xiayi Wu; Quan Liu; Ye Li; Shiyu Wu; Zhuofan Chen

doi:10.1080/14686996.2023.2186690

Optimizing the biodegradability and osteogenesis of biogenic collagen membrane via fluoride-modified polymer-induced liquid precursor process

Sci Technol Adv Mater. 2023 Mar 13;24(1):2186690. doi: 10.1080/14686996.2023.2186690. eCollection 2023.

Authors

Xiyan Li^{1

2

3}, Chuangji Li^{1

2

3}, Mengxi Su^{1

2

3}, Xinyi Zhong^{1

2

3}, Yihan Xing^{1

2

3}, Zhengjie Shan^{1

2

3}, Shoucheng Chen^{1

2

3}, Xingchen Liu^{1

2

3}, Xiayi Wu^{1

2

3}, Quan Liu^{1

2

3}, Ye Li^{1

2

3}, Shiyu Wu^{1

2

3}, Zhuofan Chen^{1

2

3}

Affiliations

¹ Hospital of Stomatology, Sun Yat-sen University, Guangzhou, China.
² Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, China.
³ Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, China.

Abstract

Biogenic collagen membranes (BCM) have been widely used in guided bone regeneration (GBR) owing to their biodegradability during tissue integration. However, their relatively high degradation rate and lack of pro-osteogenic properties limit their clinical outcomes. It is of great importance to endow BCM with tailored degradation as well as pro-osteogenic properties. In this study, a fluoride-modified polymer-induced liquid precursor (PILP) based biomineralization strategy was used to convert the collagen membrane from an organic phase to an apatite-based inorganic phase, thus achieving enhanced anti-degradation performance as well as osteogenesis. As a result, three phases of collagen membranes were prepared. The original BCM in the organic phase induced the mildest inflammatory response and was mostly degraded after 4 weeks. The organic-inorganic mixture phase of the collagen membrane evoked a prominent inflammatory response owing to the fluoride-containing amorphous calcium phosphate (F-ACP) nanoparticles, resulting in active angiogenesis and fibrous encapsulation, whereas the inorganic phase induced a mild inflammatory response and degraded the least owing to the transition of F-ACP particles into calcium phosphate with high crystallinity. Effective control of ACP is key to building novel apatite-based barrier membranes. The current results may pave the way for the development of advanced apatite-based membranes with enhanced barrier performances.

Keywords: Collagen membrane; amorphous calcium phosphate; degradation; fluoride; polymer-induced liquid precursor.

Grants and funding

This work was supported by the National Natural Science Foundation of China under grant numbers [81901055, 82001095, and 81970975]; The Guangdong Financial Fund for High-Caliber Hospital Construction.