TrypTag.org: from images to discoveries using genome-wide protein localisation in Trypanosoma brucei

Abstract

TrypTag was a 4-year project to tag the N- and C-termini of almost all Trypanosoma brucei proteins with a fluorescent protein and record the subcellular localisation through images and manual annotation. We highlight the new routes to cell biological discovery this transformative resource is enabling for parasitologists and cell biologists.

Keywords: high-throughput imaging; kinetoplastid; molecular cell biology; trypanosome.