In vitro study on how photodynamic therapy and calcium hydroxide medication influence adhesive interface properties of glass-fiber posts to intraradicular dentin

Henrico Badaoui Strazzi-Sahyon; Henrique Augusto Banci; Fernanda Santos de Melo; Lucas Silva Soares; Rubens Spin-Neto; Marco Antonio Hungaro Duarte; Luciano Tavares Angelo Cintra; Paulo Henrique Dos Santos; Gustavo Sivieri-Araujo

doi:10.1016/j.jmbbm.2023.105757

In vitro study on how photodynamic therapy and calcium hydroxide medication influence adhesive interface properties of glass-fiber posts to intraradicular dentin

J Mech Behav Biomed Mater. 2023 May:141:105757. doi: 10.1016/j.jmbbm.2023.105757. Epub 2023 Mar 5.

Authors

Henrico Badaoui Strazzi-Sahyon¹, Henrique Augusto Banci², Fernanda Santos de Melo³, Lucas Silva Soares⁴, Rubens Spin-Neto⁵, Marco Antonio Hungaro Duarte⁶, Luciano Tavares Angelo Cintra⁷, Paulo Henrique Dos Santos⁸, Gustavo Sivieri-Araujo⁹

Affiliations

¹ Department of Dental Materials and Prosthodontics, Araçatuba School of Dentistry, São Paulo State University, UNESP, Araçatuba, SP, Brazil; Department of Prosthodontics and Periodontology, Bauru School of Dentistry, University of Sao Paulo, USP, Bauru, SP, Brazil. Electronic address: ico_strazzi@usp.br.
² Department of Preventive and Restorative Dentistry, Discipline of Endodontics, Araçatuba School of Dentistry, São Paulo State University, UNESP, Araçatuba, SP, Brazil. Electronic address: henrique.banci@unesp.br.
³ Department of Preventive and Restorative Dentistry, Discipline of Endodontics, Araçatuba School of Dentistry, São Paulo State University, UNESP, Araçatuba, SP, Brazil. Electronic address: fernanda2953@hotmail.com.
⁴ Etec Araçatuba -Public School, Centro Paula Souza Araçatuba, SP, Brazil. Electronic address: lucas.ssoares249@gmail.com.
⁵ Section of Oral Radiology, Department of Dentistry and Oral Health, Aarhus University, Aarhus, Denmark. Electronic address: rsn@dent.au.dk.
⁶ Department of Restorative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, São Paulo University, USP, Bauru, SP, Brazil. Electronic address: mhungaro@fob.usp.br.
⁷ Department of Preventive and Restorative Dentistry, Discipline of Endodontics, Araçatuba School of Dentistry, São Paulo State University, UNESP, Araçatuba, SP, Brazil. Electronic address: luciano.cintra@unesp.br.
⁸ Department of Dental Materials and Prosthodontics, Araçatuba School of Dentistry, São Paulo State University, UNESP, Araçatuba, SP, Brazil; Dental Research Institute, University of Toronto, Faculty of Dentistry, Toronto, ON, Canada. Electronic address: pauloh.dossantos@utoronto.ca.
⁹ Department of Preventive and Restorative Dentistry, Discipline of Endodontics, Araçatuba School of Dentistry, São Paulo State University, UNESP, Araçatuba, SP, Brazil. Electronic address: gustavo.sivieri@unesp.br.

PMID: 36924612
DOI: 10.1016/j.jmbbm.2023.105757

Abstract

Purpose: This in vitro study evaluated the influence of antimicrobial photodynamic therapy (aPDT), using methylene blue (MB) as photosensitizer (PS) and calcium hydroxide (CH) as intracanal medication on adhesive bond strength, sealing, and integrity of the luting interface of glass-fiber posts to different thirds of endodontically treated root canal dentin.

Material and methods: 102 incisors were sorted into 6 groups: a negative control irrigated with deionized water; a positive control irrigated with deionized water and filled with CH; CH + MB 50 mg/L without laser radiation; CH + MB 100 mg/L without laser radiation; CH + MB 50 mg/L radiated by red laser; and CH + MB 100 mg/L radiated by red laser. Push-out bond strength (n = 8), adhesive interface sealing (n = 3), and volume and quantification of voids (n = 6) were assessed using a universal testing machine, confocal laser scanning microscope, and computerized microtomography, respectively. Scanning electron micrographs were obtained from representative samples to qualify the fracture patterns. Push-out bond strength and adhesive interface integrity data were subjected to 2-way ANOVA for repeated measures followed by Tukey's test (α = 0.05). Adhesive interface sealing was evaluated by the inter-examiner Kappa test and submitted to Kruskal-Wallis and Dunns tests (α = 0.05).

Results: Assessing the apical region, the positive control and MB_100WA + Ca(OH)₂ groups showed lower adhesive bond strength compared to the MB_100A + Ca(OH)₂ group (P < 0.05). The cervical third showed higher bond strength than the apical third for the positive control, MB_50WA + Ca(OH)_2, MB_100WA + Ca(OH)_2, and MB_50A + Ca(OH)₂ groups (P < 0.05). A prevalence of mixed failure was observed in all experimental groups. There were no statistically significant differences in adhesive interface sealing for any of the parameters assessed (P > 0.05). MB_100WA + Ca(OH)₂ and MB_100A + Ca(OH)₂ groups promoted a higher volume and quantification of voids compared to the negative control group evaluating the cervical third (P < 0.05). In general, there were no differences in the quantification of voids comparing the intraradicular thirds (P > 0.05), differently to the volume of voids in which, in general, the cervical third promoted higher values compared to the middle and apical thirds (P < 0.05).

Conclusion: aPDT with methylene blue PS at 50 mg/L associated with calcium hydroxide as intracanal medication demonstrated satisfactory bond strength, sealing, and integrity of the adhesive interface at any intraradicular depth.

Keywords: Bond strength; Calcium hydroxide; Dentin; Methylene blue; Photodynamic therapy; Resin cements.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adhesives
Calcium Hydroxide
Dental Bonding*
Dentin
Glass / chemistry
Materials Testing
Methylene Blue
Photochemotherapy* / methods
Resin Cements / chemistry
Water

Substances

Calcium Hydroxide
Adhesives
Methylene Blue
Water
Resin Cements