A method for optimizing an automatic selection of values for parameters that feed segmentation algorithms is proposed. Evolutionary optimization techniques in combination with a fitness function based on a mutual information parameter have been used to find the optimal parameter values of region growing, fuzzy c-means and graph cut segmentation algorithms. To validate the method, the segmentation of two transmission electron microscopy tomography reconstructed volumes of a carbon black-reinforced rubber and a polylactic acid and clay nanocomposite is carried out (i) using evolutionary optimization techniques and (ii) manually by experts. The results confirm that the use of evolutionary optimization techniques, such as genetic algorithms, reduces the computational operation cost needed for a total grid search of segmentation parameters, reducing the probability of reaching a false optimum, and improving the segmentation quality. HIGHLIGHTS: A new approach to optimize 3D segmentation algorithms. Methodology to optimize segmentation parameters and improve segmentation quality. Improvement on the results when using region growing, fuzzy c-means and graph cuts algorithms.
Keywords: TEM tomography; characterization; genetic algorithms; microscopy; mutual information; optimization techniques; segmentation; segmentation algorithms.
© 2023 The Authors. Microscopy Research and Technique published by Wiley Periodicals LLC.