Biopesticide efficacy of four plant essential oils against papaya mealybug, Paracoccus marginatus Williams and Granara de Willink (Hemiptera: Pseudococcidae)

Regina W Mwanauta; Patrick A Ndakidemi; Pavithravani B Venkataramana

doi:10.1016/j.heliyon.2023.e14162

Biopesticide efficacy of four plant essential oils against papaya mealybug, Paracoccus marginatus Williams and Granara de Willink (Hemiptera: Pseudococcidae)

Heliyon. 2023 Mar 1;9(3):e14162. doi: 10.1016/j.heliyon.2023.e14162. eCollection 2023 Mar.

Authors

Regina W Mwanauta^{1

2}, Patrick A Ndakidemi¹, Pavithravani B Venkataramana¹

Affiliations

¹ School of Life Sciences and Bioengineering (LiSBE), Nelson Mandela African Institution of Science and Technology, P.O. Box 447, Arusha, Tanzania.
² Tanzania Plant Health and Pesticides Authority (TPHPA), P.O BOX 3024, Arusha, Tanzania.

Abstract

Worldwide, P. marginatus causes 75% of estimated economic loss in papaya farming, with an increase in production costs. The extract of plant essential oils (PEO) has the potential to control P. marginatus by degrading its wax coatings to death; however, it is less studied in the East African agroecosystem. Therefore, this study was conducted to evaluate the efficacy of four PEO from (neem, citrus, garlic, and castor) against P. marginatus at different concentrations (0.5%, 1%, and 1.5%) with and without 0.2% adjuvants separately as a biopesticide. The experiment was conducted in a completely randomized design with four replications per treatment concentration. The papaya seeds (Carina variety) were used in the experiment. After 3 weeks from transplanting, 50 P. marginatus specimens were inoculated in each plant. Before treatment application, insect abundance, leaf curling, yellowing, and soot mold were assessed. Then, 24 h, 48 h, and 72 h after biopesticide application, insect mortality was assessed. The results showed a significant difference (p = 0.001) for all assessment intervals in PEOs. However, for the PEOs in combination with the adjuvants, the results were significantly different (p = 0.001) only at 24 h. It was found that among the biopesticides, neem oil (1.5%) + isopropyl alcohol was highly effective (95.5%) after 72 h followed by (Imidacloprid (91%), citrus oil 1.5% (90.7%) and neem oil (1.5%) + paraffin oil (81.0%). But also, there were significant differences among treatments on leaf curling, yellowing, and soot mold reduction in papaya plants 21 days after spray. We conclude that neem oil (1.5%) + 0.2% isopropyl alcohol, neem oil (1.5%) + paraffin oil, and citrus oil (1.5%) significantly controlled P. marginatus. Thus, we recommend adopting these formulations for papaya farmers to control P. marginatus in their farms; however, simple formulations which can be easily accessed by smallholder farmers are essential.

Keywords: Adjuvants; Botanical pesticides; Carica papaya; Invasive pests; Pest management.