A rapid extraction process monitoring of Swertia mussotii Franch. With near infrared spectroscopy

Mengqi Zhang; Bing Zhao; Lian Li; Lei Nie; Peipei Li; Jing Sun; Aoli Wu; Hengchang Zang

doi:10.1016/j.saa.2023.122609

A rapid extraction process monitoring of Swertia mussotii Franch. With near infrared spectroscopy

Spectrochim Acta A Mol Biomol Spectrosc. 2023 Jul 5:295:122609. doi: 10.1016/j.saa.2023.122609. Epub 2023 Mar 11.

Authors

Mengqi Zhang¹, Bing Zhao¹, Lian Li¹, Lei Nie¹, Peipei Li², Jing Sun², Aoli Wu¹, Hengchang Zang³

Affiliations

¹ National Medical Products Administration Key Laboratory for Technology Research and Evaluation of Drug Products, School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, China.
² Qinghai Provincial Key Laboratory of Qinghai-Tibet Plateau Biological Resources, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai, 810008, China.
³ National Medical Products Administration Key Laboratory for Technology Research and Evaluation of Drug Products, School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, China; National Glycoengineering Research Center, Shandong University, Jinan, Shandong, 250012, China; Key Laboratory of Chemical Biology (Ministry of Education), Shandong University, Jinan, Shandong, 250012, China. Electronic address: zanghcw@126.com.

PMID: 36921517
DOI: 10.1016/j.saa.2023.122609

Abstract

Swertia mussotii Franch. (SMF), a traditional Tibetan medicine, which has miraculous effect on treating hepatitis diseases. However, there is no research on its entire production process, and invisible production process has seriously hindered the SMF modern development. In this study, principal component analysis (PCA), subtractive spectroscopy, and two-dimensional correlation spectroscopy (2D-COS) were used to explain changes of characteristic groups in the extraction process. Four main characteristic peaks at 1884 nm, 1944 nm, 2246 nm and 2308 nm were identified to describe the changes of molecular structure information of total active components in SMF extraction process. In addition, multi critical quality attributes (CQAs) models were established by near-infrared spectroscopy (NIRS) combined with the total quantum statistical moment (TQSM). The coefficients of determination (R²_eval and R²_ival) were both greater than 0.99. The ratios of the standard deviation of validation to the standard error of the prediction (RPD_e and RPD_i) were greater than five. The quantitative model of AUC_T could save time on primary data measurement by not requiring determination of indicator components compared with others. In conclusion, these results demonstrated that it was feasible to understand the SMF extraction process through AUC_T and characteristic groups. These could realize the visual digital characterization and quality stability of the SMF extraction process.

Keywords: Extraction process; Near-infrared spectroscopy; Swertia mussotii Franch.; Tibetan medicine; Total quantum statistical moment.

MeSH terms

Spectroscopy, Near-Infrared
Swertia* / chemistry