Pseudotyped Viruses for Phlebovirus

Jiajing Wu; Weijin Huang; Youchun Wang

doi:10.1007/978-981-99-0113-5_13

Pseudotyped Viruses for Phlebovirus

Adv Exp Med Biol. 2023:1407:253-264. doi: 10.1007/978-981-99-0113-5_13.

Authors

Jiajing Wu¹, Weijin Huang², Youchun Wang^{3

4}

Affiliations

¹ Beijing Yunling Biotechnology Co. Ltd, Beijing, China.
² Division of HIV/AIDS and Sex-transmitted Virus Vaccines, National Institutes for Food and Drug Control (NIFDC) and WHO Collaborating Center for Standardization and Evaluation of Biologicals, Beijing, China.
³ Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. wangyc@nifdc.org.cn.
⁴ Institute of Medical Biology, Chinese Academy of Medicine Sciences & Peking Union Medical College, Kunming, China. wangyc@nifdc.org.cn.

PMID: 36920701
DOI: 10.1007/978-981-99-0113-5_13

Abstract

Rift Valley fever virus (RVFV) is a member of the Phlebovirus genus, one of the 20 genera in the Phenuiviridae family. RVFV causes disease in animals and humans and is transmitted by sandflies or ticks. However, research into RVFV is limited by the requirement for biosafety level 3 (BSL-3) containment. Pseudotyped virus overcomes this limitation as it can be handled in a BSL-2 environment. Pseudotyped RVFV possesses an identical envelope protein structure to that of the authentic virus, simulating the same process of receptor binding and membrane fusion to host cells. Pseudotyped phleboviruses are therefore useful tools to study the infection mechanism of these viruses and for the screening of inhibitory drugs and the development of therapeutic monoclonal antibodies.

Keywords: In vitro; In vivo; Neutralization; Pseudotyped virus; Rift Valley fever virus.

MeSH terms

Animals
Humans
Phlebovirus* / genetics
Rift Valley Fever* / prevention & control
Rift Valley fever virus* / genetics
Viral Pseudotyping