Pseudotyped Viruses for Enterovirus

Xing Wu; Lisha Cui; Yu Bai; Lianlian Bian; Zhenglun Liang

doi:10.1007/978-981-99-0113-5_11

Pseudotyped Viruses for Enterovirus

Adv Exp Med Biol. 2023:1407:209-228. doi: 10.1007/978-981-99-0113-5_11.

Authors

Xing Wu^{1

2}, Lisha Cui³, Yu Bai^{1

2}, Lianlian Bian^{1

2}, Zhenglun Liang^{1

2}

Affiliations

¹ Division of Hepatitis Virus & Enterovirus Vaccines, Institute for Biological Products, National Institutes for Food and Drug Control, Beijing, China.
² WHO Collaborating Center for Standardization and Evaluation of Biologicals, Beijing, China.
³ Minhai biotechnology Co. Ltd, Beijing, China.

PMID: 36920699
DOI: 10.1007/978-981-99-0113-5_11

Abstract

Using a non-pathogenic pseudotyped virus as a surrogate for a wide-type virus in scientific research complies with the recent requirements for biosafety. Enterovirus (EV) contains many species of viruses, which are a type of nonenveloped virus. The preparation of its corresponding pseudotyped virus often needs customized construction compared to some enveloped viruses. This article describes the procedures and challenges in the construction of pseudotyped virus for enterovirus (pseudotyped enterovirus, EVpv) and also introduces the application of EVpv in basic virological research, serological monitoring, and the detection of neutralizing antibody (NtAb).

Keywords: Enterovirus; Neutralizing antibody; Pseudotyped virus.

MeSH terms

Antibodies, Neutralizing
Antigens, Viral
Enterovirus Infections*
Enterovirus* / genetics
Humans
Viral Pseudotyping

Substances

Antibodies, Neutralizing
Antigens, Viral