Trichoderma metabolites trigger aggregation behavior in Formosan subterranean termites (Coptotermes formosanus)

Abstract

Our previous studies have shown that some Trichoderma fungi trigger aggregation behavior in Formosan subterranean termites, Coptotermes formosanus Shiraki. However, the mechanisms underlying the induction of termite aggregation by Trichoderma fungi remain unclear. Here, we found that the aqueous or acetone extract of Trichoderma asperellum Samuels, Lieckfeldt & Nirenberg and Trichoderma virens Pers. ex Fries isolated from the gut or cuticle of C. formosanus elicited significant termite aggregation in 2-choice tests. We then screened 9 Trichoderma metabolites (3-acetoxy-2-butanone, phenol, 3-ethoxypropionic acid, ethyl 2,4-dioxovalerate, diglycolic acid, d-valine, styrene, 3-aminopyridine, and hexyl acetoacetate) that triggered termite aggregation. Among them, phenol (1 000 μg/mL), 3-ethoxypropionic acid (10 μg/mL), ethyl 2,4-dioxovalerate (1 000 μg/mL), and diglycolic acid (1 000 μg/mL) showed the strongest activities, triggering termite aggregation throughout the 24-h period. As T. asperellum and T. virens produce different metabolites that trigger aggregation behavior in termites, the mechanisms underlying the interaction between subterranean termites and Trichoderma fungi likely vary. Future studies are needed to test whether these chemicals can attract termites and increase bait consumption.

Keywords: Trichoderma; aggregation behavior; insect-fungus interaction; semiochemical; subterranean termite.