Multilayered blow-spun vascular prostheses with luminal surfaces in Nano/Micro range: the influence on endothelial cell and platelet adhesion

Iwona Łopianiak; Wiktoria Rzempołuch; Mehtap Civelek; Iwona Cicha; Tomasz Ciach; Beata A Butruk-Raszeja

doi:10.1186/s13036-023-00337-9

Multilayered blow-spun vascular prostheses with luminal surfaces in Nano/Micro range: the influence on endothelial cell and platelet adhesion

J Biol Eng. 2023 Mar 13;17(1):20. doi: 10.1186/s13036-023-00337-9.

Authors

Iwona Łopianiak^{1

2}, Wiktoria Rzempołuch¹, Mehtap Civelek³, Iwona Cicha³, Tomasz Ciach^{1

4}, Beata A Butruk-Raszeja⁵

Affiliations

¹ Faculty of Chemical and Process Engineering, Warsaw University of Technology, Waryńskiego 1, 00-645, Warsaw, Poland.
² Doctoral School of Warsaw University of Technology, Warsaw University of Technology, Pl. Politechniki 1, 00-661, Warsaw, Poland.
³ Section of Experimental Oncology Und Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung-Professorship, ENT-Department, Universitätsklinikum, Erlangen, Germany.
⁴ Centre for Advanced Materials and Technologies CEZAMAT, Warsaw University of Technology, Poleczki 19, 02-822, Warsaw, Poland.
⁵ Faculty of Chemical and Process Engineering, Warsaw University of Technology, Waryńskiego 1, 00-645, Warsaw, Poland. Beata.Raszeja@pw.edu.pl.

Abstract

Background: In this study, two types of polyurethane-based cylindrical multilayered grafts with internal diameters ≤ 6 mm were produced by the solution blow spinning (SBS) method. The main aim was to create layered-wall prostheses differing in their luminal surface morphology. Changing the SBS process parameters, i.e. working distance, rotational speed, volume, and concentration of the polymer solution allowed to obtain structures with the required morphologies. The first type of prostheses, termed Nano, possessed nanofibrous luminal surface, and the second type, Micro, presented morphologically diverse luminal surface, with both solid and microfibrous areas.

Results: The results of mechanical tests confirmed that designed prostheses had high flexibility (Young's modulus value of about 2.5 MPa) and good tensile strength (maximum axial load value of about 60 N), which meet the requirements for vascular prostheses. The influence of the luminal surface morphology on platelet adhesion and the attachment of endothelial cells was investigated. Both surfaces did not cause hemolysis in contact with blood, the percentage of platelet-occupied area for Nano and Micro surfaces was comparable to reference polytetrafluoroethylene (PTFE) surface. However, the change in morphology of surface-adhered platelets between Nano and Micro surfaces was visible, which might suggest differences in their activation level. Endothelial coverage after 1, 3, and 7 days of culture on flat samples (2D model) was higher on Nano prostheses as compared with Micro scaffolds. However, this effect was not seen in 3D culture, where cylindrical prostheses were colonized using magnetic seeding method.

Conclusions: We conclude the produced scaffolds meet the material and mechanical requirements for vascular prostheses. However, changing the morphology without changing the chemical modification of the luminal surface is not sufficient to achieve the appropriate effectiveness of endothelialization in the 3D model.

Keywords: Endothelial cells; Hemocompatibility; Magnetic seeding; Multilayered small-diameter vascular grafts; Nanofibers; Solution blow spinning.

Abstract

Grants and funding