Relationship between interferon-induced transmembrane protein 3 and matrix metalloproteinase-9 gene polymorphisms in patients with hepatocellular carcinoma

Lamiaa A Barakat; Alyaa R Elsergany; Maivel H Ghattas; Nancy Mahsoub; Rania M Bondok

doi:10.1016/j.clinre.2023.102110

Relationship between interferon-induced transmembrane protein 3 and matrix metalloproteinase-9 gene polymorphisms in patients with hepatocellular carcinoma

Clin Res Hepatol Gastroenterol. 2023 May;47(5):102110. doi: 10.1016/j.clinre.2023.102110. Epub 2023 Mar 11.

Authors

Lamiaa A Barakat¹, Alyaa R Elsergany², Maivel H Ghattas³, Nancy Mahsoub⁴, Rania M Bondok⁵

Affiliations

¹ Department of Biochemistry, Faculty of Science, Port-Said University, Egypt. Electronic address: lamiaabarakat1@gmail.com.
² Internal Medicine Department , Oncology Center, Faculty of Medicine, Mansoura University, Egypt.
³ Department of Medical Biochemistry, Faculty of Medicine, Port-Said University, Egypt.
⁴ Department of Clinical Pathology, Faculty of Medicine, Mansoura University, Egypt.
⁵ Department of Biochemistry, Faculty of Science, Port-Said University, Egypt.

PMID: 36914067
DOI: 10.1016/j.clinre.2023.102110

Abstract

Background: Hepatocellular carcinoma originates from hepatocytes as a result of the effects of numerous genetic variations. Interferon-Induced Transmembrane protein 3 (IFITM3) is involved in the processes of cellular differentiation, apoptosis, cell adhesion, and immune cell regulation. Matrix Metalloproteinase-9 (MMP-9) are zinc dependent endopeptidases that cleave extracellular matrix contents and play an important role in the progression of cancer.

Objective: The study aimed to outline the key molecular biology progression in hepatocellular carcinoma and the relationship between hepatocellular cancer and genetic polymorphisms of IFITM3 and MMP-9.

Methods: In total 200 patients with hepatocellular carcinoma patients (n=100) and a control group with Hepatitis C virus (n=100) which collected randomly from the EL-Mansoura oncology center during the interval between June 2020 and October 2021. The expression of MMP-9 and the IFITM3 SNP was investigated. MMP-9 gene polymorphisms were estimated by using PCR-RFLP and IFITM3 gene was detected using DNA sequencing, ELISA was used to measure protein levels of MMP-9 and IFITM3.

Results: The T allele of MMP-9 was more frequent among patients (n=121) than control subjects (n=71). The C allele of IFITM3 was more frequent among patients (n=112) than control subjects (n=83), polymorphisms of the genes linked to a high risk of disease development, patients of MMP-9 (TT genotype), odd ratio (OR) = 2.63, IFITM3 (CC genotype), OR= 2.43.

Conclusions: We found that the genetic polymorphisms of MMP-9 and IFITM3 are related to the occurrence and development of hepatocellular carcinoma. This study might be utilized in clinical diagnosis and therapy and to provide a baseline for prevention.

Keywords: Gene polymorphisms; Hepatitis C virus; Hepatocellular carcinoma; Interferon-induced transmembrane protein-3; Matrix metalloproteinase-9.

MeSH terms

Carcinoma, Hepatocellular* / genetics
Genetic Predisposition to Disease
Genotype
Humans
Interferons
Liver Neoplasms* / genetics
Liver Neoplasms* / pathology
Matrix Metalloproteinase 9* / genetics
Membrane Proteins / genetics
Polymorphism, Single Nucleotide
RNA-Binding Proteins* / genetics

Substances

IFITM3 protein, human
Interferons
Matrix Metalloproteinase 9
Membrane Proteins
RNA-Binding Proteins