Comparison of the diversity and structure of the rhizosphere microbial community between the straight and twisted trunk types of Pinus yunnanensis

Peiling Li; Dan Zong; Peihua Gan; Hailin Li; Zhiyang Wu; Fahong Li; Changlin Zhao; Laigeng Li; Chengzhong He

doi:10.3389/fmicb.2023.1066805

Comparison of the diversity and structure of the rhizosphere microbial community between the straight and twisted trunk types of Pinus yunnanensis

Front Microbiol. 2023 Feb 23:14:1066805. doi: 10.3389/fmicb.2023.1066805. eCollection 2023.

Authors

Peiling Li^{1

2}, Dan Zong^{1

2

3}, Peihua Gan^{1

2}, Hailin Li^{1

2}, Zhiyang Wu^{1

2}, Fahong Li^{1

2}, Changlin Zhao^{3

4}, Laigeng Li⁵, Chengzhong He^{1

2

3}

Affiliations

¹ Key Laboratory for Forest Genetics and Tree Improvement and Propagation in Universities of Yunnan, Southwest Forestry University, Kunming, China.
² Key Laboratory of Biodiversity Conservation in Southwest China, State Forestry Administration, Southwest Forestry University, Kunming, China.
³ Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming, China.
⁴ College of Biodiversity Conservation, Southwest Forestry University, Kunming, Yunnan, China.
⁵ National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China.

Abstract

Background: Pinus yunnanensis is a major silvicultural species in Southwest China. Currently, large areas of twisted-trunk Pinus yunnanensis stands severely restrict its productivity. Different categories of rhizosphere microbes evolve alongside plants and environments and play an important role in the growth and ecological fitness of their host plant. However, the diversity and structure of the rhizosphere microbial communities between P. yunnanensis with two different trunk types-straight and twisted-remain unclear.

Methods: We collected the rhizosphere soil of 5 trees with the straight and 5 trees with the twisted trunk type in each of three sites in Yunnan province. We assessed and compared the diversity and structure of the rhizosphere microbial communities between P. yunnanensis with two different trunk types by Illumina sequencing of 16S rRNA genes and internal transcribed spacer (ITS) regions.

Results: The available phosphorus in soil differed significantly between P. yunnanensis with straight and twisted trunks. Available potassium had a significant effect on fungi. Chloroflexi dominated the rhizosphere soils of the straight trunk type, while Proteobacteria was predominant in the rhizosphere soils of the twisted trunk type. Trunk types significantly explained 6.79% of the variance in bacterial communities.

Conclusion: This study revealed the composition and diversity of bacterial and fungal groups in the rhizosphere soil of P. yunnanensis with straight and twisted trunk types, providing proper microbial information for different plant phenotypes.

Keywords: microbial community; microbial diversity; pinus yunnanensis; rhizosphere microorganism; trunk type.

Grants and funding

This work was supported by Yunnan Provincial Expert Workstation (202005AF150020), Yunnan Provincial “Ten-Thousand Program” for Leading Industry Innovation (YUWR-CYJS-2020-018), and Applied Basic Research Programs of Yunnan Province of China (2017FA011).