SERS-based immunomagnetic bead for rapid detection of H5N1 influenza virus

Influenza Other Respir Viruses. 2023 Mar 9;17(3):e13114. doi: 10.1111/irv.13114. eCollection 2023 Mar.

Abstract

The surface-enhanced Raman scattering (SERS) has recently drawn attention in the detection of respiratory viruses, but there have been few reports of the direct detection of viruses. In this study, a sandwich immunomagnetic bead SERS was established for the rapid diagnosis of the H5N1 influenza virus. The detection limit was estimated to be 5.0 × 10-6 TCID50/ml. The method showed excellent specificity with no cross-reaction with H1N1, H5N6 or H9N2. The H5N1 influenza virus detection accuracy of the SERS method was 100% in chicken embryos. The results hold great promise for the utilization of SERS as an innovative approach in the diagnosis of influenza virus.

Keywords: H5N1 influenza virus; SERS; label‐free.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chick Embryo
  • Chickens
  • Humans
  • Influenza A Virus, H1N1 Subtype*
  • Influenza A Virus, H5N1 Subtype*
  • Influenza A Virus, H9N2 Subtype*
  • Influenza in Birds*
  • Influenza, Human*