A Tumor-admixture Model to Interrogate Immune Cell-dependent Tumorigenesis

Jordan T Noe; Chuanlin Ding; Anne E Geller; Beatriz E Rendon; Jun Yan; Robert A Mitchell

doi:10.21769/BioProtoc.4630

A Tumor-admixture Model to Interrogate Immune Cell-dependent Tumorigenesis

Bio Protoc. 2023 Mar 5;13(5):e4630. doi: 10.21769/BioProtoc.4630.

Authors

Jordan T Noe^{1

2}, Chuanlin Ding^{3

2

4}, Anne E Geller^{3

2}, Beatriz E Rendon², Jun Yan^{3

2

4}, Robert A Mitchell^{1

3

2

4}

Affiliations

¹ Department of Biochemistry and Molecular Genetics, University of Louisville, Louisville, KY 40202, USA.
² J.G. Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA.
³ Department of Microbiology and Immunology, University of Louisville, Louisville, KY 40202, USA.
⁴ Department of Surgery, Division of Immunotherapy, University of Louisville, Louisville, KY 40202, USA.

Abstract

A rigorous determination of effector contributions of tumor-infiltrating immune cells is critical for identifying targetable molecular mechanisms for the development of novel cancer immunotherapies. A tumor/immune cell-admixture model is an advantageous strategy to study tumor immunology as the fundamental methodology is relatively straightforward, while also being adaptable to scale to address increasingly complex research queries. Ultimately, this method can provide robust experimental information to complement more traditional murine models of tumor immunology. Here, we describe a tumor/macrophage-admixture model using bone marrow-derived macrophages to investigate macrophage-dependent tumorigenesis. Additionally, we provide commentary on potential branch points for optimization with other immune cells, experimental techniques, and cancer types.

Keywords: Cancer; Immunology; Immunotherapy; Macrophage; Mouse models.

Abstract

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