Anti-inflammatory effects of fish bone fermented using Monascus purpureus in LPS-induced RAW264.7 cells by regulating NF-κB pathway

Ya-Ting Chen; Shu-Jen Chen; Li-Jung Yin; Chun-Yi Hu; Cheng-Di Dong; Reeta Rani Singhania; Shu-Ling Hsieh

doi:10.1007/s13197-022-05413-4

Anti-inflammatory effects of fish bone fermented using Monascus purpureus in LPS-induced RAW264.7 cells by regulating NF-κB pathway

J Food Sci Technol. 2023 Mar;60(3):958-965. doi: 10.1007/s13197-022-05413-4. Epub 2022 Mar 12.

Authors

Ya-Ting Chen¹, Shu-Jen Chen², Li-Jung Yin¹, Chun-Yi Hu³, Cheng-Di Dong⁴, Reeta Rani Singhania⁴, Shu-Ling Hsieh^{1

5}

Affiliations

¹ Department of Seafood Science, National Kaohsiung University of Science and Technology, Kaohsiung, 81157 Taiwan.
² Department of Chemical and Materials Engineering, National Kaohsiung University of Science and Technology, Kaohsiung, 80778 Taiwan.
³ Department of Food Science and Nutrition, Meiho University, Pingtung, 912009 Taiwan.
⁴ Department of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung, 81157 Taiwan.
⁵ Department of Seafood Science, National Kaohsiung University of Science and Technology, 142 Haijhuan Rd, Nanzih District, Kaohsiung City, 81143 Taiwan.

Abstract

Fish bones are the by-products of aquatic and fishery processing, which are often discarded. However, it has been considered having health-promoting by containing many essential nutrients. This study investigates the anti-inflammatory effect of fish bone fermented by Monascus purpureus (FBF) and the NF-κB pathway regulation mechanism in lipopolysaccharides (LPS)-induced RAW 264.7 cells. FBF has inhibited the production of PGE₂ (prostaglandin E₂), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in LPS-induced RAW264.7 cells. The FBF has significantly inhibited mRNA expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Moreover, FBF has suppressed activation of NF-κB (nuclear factor kappa-B) by increasing IκB mRNA expression and reduced of p65, p50 mRNA expression, as well as nuclear NF-κB DNA binding activity in LPS-induced RAW 246.7 cells. These findings demonstrate that FBF has inhibited LPS-induced inflammation by subsiding the activation of NF-κB in RAW 246.7 cells, implying that FBF could be employed as a promising natural product.

Keywords: Anti-inflammatory; Fermentation; Fish bone; Monascus purpureus; NF-κB.