Effects of chronic low-dose aflatoxin B1 exposure in lactating Florida dairy goats

Abstract

In the past few years there has been a growing trend in the prevalence of aflatoxins, attributable to climate change, in substances destined for animal feeding, together with an increase in dairy product consumption. These facts have triggered great concern in the scientific community over milk pollution by aflatoxin M₁. Therefore, our study aimed to determine the transfer of aflatoxin B₁ from the diet into milk as AFM₁ in goats exposed to different concentrations of AFB₁, and its possible effect on the production and serological parameters of this species. For this purpose, 18 goats in late lactation were divided into 3 groups (n = 6) and exposed to different daily doses of aflatoxin B₁ (T1 = 120 µg; T2 = 60 µg, and control = 0 µg), during 31 d. Pure aflatoxin B₁ was administered 6 h before each milking in an artificially contaminated pellet. The milk samples were taken individually in sequential samples. Milk yield and feed intake were recorded daily, and a blood sample was extracted on the last day of exposure. No aflatoxin M₁ was detected, either in the samples taken before the first administration, or in the control group ones. The aflatoxin M₁ concentration detected in the milk (T1 = 0.075 µg/kg; T2 = 0.035 µg/kg) increased significantly on a par with the amount of aflatoxin B₁ ingested. The amount of aflatoxin B₁ ingested did not have any influence on aflatoxin M₁ carryover (T1 = 0.066% and T2 = 0.060%), these being considerably lower than those described in dairy goats. Thus, we concluded that the concentration of aflatoxin M₁ in milk follows a linear relationship with respect to the aflatoxin B₁ ingested, and that the aflatoxin M₁ carryover was not affected by the administration of different aflatoxin B₁ doses. Similarly, no significant changes in the production parameters after chronic exposure to aflatoxin B₁ were observed, revealing a certain resistance of the goat to the possible effects of that aflatoxin.

Keywords: aflatoxin B(1); aflatoxin M(1); carryover; mycotoxins.