Transcriptomic analysis of entomopathogenic fungus Beauveria bassiana infected by a hypervirulent polymycovirus BbPmV-4

Qin Kang; Siyu Ning; Li Sui; Yang Lu; Yu Zhao; Wangpeng Shi; Qiyun Li; Zhengkun Zhang

doi:10.1016/j.funbio.2023.02.003

Transcriptomic analysis of entomopathogenic fungus Beauveria bassiana infected by a hypervirulent polymycovirus BbPmV-4

Fungal Biol. 2023 Mar;127(3):958-967. doi: 10.1016/j.funbio.2023.02.003. Epub 2023 Feb 20.

Authors

Qin Kang¹, Siyu Ning¹, Li Sui², Yang Lu², Yu Zhao², Wangpeng Shi³, Qiyun Li⁴, Zhengkun Zhang⁵

Affiliations

¹ Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Jilin Key Laboratory of Agricultural Microbiology, Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Areas, Changchun, 130033, Jilin Province, PR China; Department of Entomology, College of Plant Protection, China Agricultural University, Beijing, 100193, PR China.
² Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Jilin Key Laboratory of Agricultural Microbiology, Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Areas, Changchun, 130033, Jilin Province, PR China.
³ Department of Entomology, College of Plant Protection, China Agricultural University, Beijing, 100193, PR China. Electronic address: wpshi@cau.edu.cn.
⁴ Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Jilin Key Laboratory of Agricultural Microbiology, Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Areas, Changchun, 130033, Jilin Province, PR China; Department of Entomology, College of Plant Protection, China Agricultural University, Beijing, 100193, PR China. Electronic address: qyli1225@126.com.
⁵ Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Jilin Key Laboratory of Agricultural Microbiology, Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Areas, Changchun, 130033, Jilin Province, PR China. Electronic address: zzk1980@cjaas.com.

PMID: 36906386
DOI: 10.1016/j.funbio.2023.02.003

Abstract

Polymycoviridae is a recently established family of mycoviruses. Beauveria bassiana polymycovirus 4 (BbPmV-4) was previously reported. However, the effect of the virus on host fungus B. bassiana was not clarified. Here, a comparison between virus-free and virus-infected isogenic lines of B. bassiana revealed that BbPmV-4 infection of B. bassiana changes morphology and could lead to decreases in conidiation and increases in virulence against Ostrinia furnacalis larvae. The differential expression of genes between virus-free and virus-infected strains was compared by RNA-Seq and was consistent with the phenotype of B. bassiana. The enhanced pathogenicity may be related to the significant up-regulation of genes encoding mitogen activated protein kinase, cytochrome P450, and polyketide synthase. The results enable studies of the mechanism of interaction between BbPmV-4 and B. bassiana.

Keywords: Beauveria bassiana polymycovirus 4; Entomopathogenic fungi; Hypervirulence; Transcriptome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Beauveria*
Gene Expression Profiling
Moths* / microbiology
Transcriptome
Virulence