Inflammation-related mRNA expression in patients with multiple myeloma undergoing hematopoietic stem cell mobilization

Abstract

Mobilization of CD34+ cells is a key element in the therapy of patients with multiple myeloma (MM) undergoing autologous stem cell transplantation. The use of chemotherapy and the granulocyte colony-stimulating factor can significantly affect the expression of inflammation-related proteins and the migration of hematopoietic stem cells. We assessed the mRNA expression of selected proteins involved in the inflammatory landscape in patients with MM (n = 71). The study aimed to evaluate C-C motif chemokine ligands 3, 4, 5 (CCL3, CCL4, CCL5), leukocyte cell-derived chemotaxin 2 (LECT2), tumor necrosis factor (TNF), and formyl peptide receptor 2 (FPR2) levels in the course of mobilization and their role in the CD34+ collection efficacy. mRNA expression from peripheral blood (PB) plasma was evaluated by Reverse transcription polymerase chain reaction. We observed a deep decline in CCL3, CCL4, LECT2, and TNF mRNA expression on the day of the first apheresis (day A) compared with that at baseline. A negative correlation was observed between CCL3, FPR2, LECT2, TNF level, and the CD34+ cells count in PB on day A, and the number of CD34+ cells obtained at first apheresis. Our results indicate that the investigated mRNAs significantly alter and may regulate the migration of CD34+ cells during mobilization. Moreover, in the case of FPR2 and LECT2, the results obtained in patients differed from the murine models.