Longitudinal tracking of T cell lymphomas in mice using flow cytometry

Elizabeth A Kuczynski; Larissa Carnevalli; Charles Sinclair

doi:10.1016/j.xpro.2023.102144

Longitudinal tracking of T cell lymphomas in mice using flow cytometry

STAR Protoc. 2023 Mar 9;4(2):102144. doi: 10.1016/j.xpro.2023.102144. Online ahead of print.

Authors

Elizabeth A Kuczynski¹, Larissa Carnevalli², Charles Sinclair³

Affiliations

¹ AstraZeneca, Cambridge CB2 0AA, UK. Electronic address: elizabeth.kuczynski@astrazeneca.com.
² AstraZeneca, Cambridge CB2 0AA, UK.
³ Flagship Pioneering, Suite 500E, 55 Cambridge Parkway, Cambridge, MA 02142, USA. Electronic address: csinclair@flagshippioneering.com.

Abstract

T cell hematological cancer has a complex interplay with host immune cells, but the ability to experimentally discriminate transferred cancer cells from host cells by flow cytometry is technically challenging. Here, we present a flow cytometry protocol to evaluate cancer cell and host immune phenotypes following transplant of a T cell lymphoma bearing a congenic marker (CD45.2) into a syngeneic host (CD45.1). We describe steps for isolation of primary immune cells from mice, staining preparation with flow cytometry antibody cocktails, and analysis by flow cytometry. For complete details on the use and execution of this protocol, please refer to Kuczynski et al.¹.

Keywords: Cancer; Cell isolation; Flow Cytometry/Mass Cytometry; Immunology.