The quite low cross-reactivity of Kawatsu's anti-tetrodotoxin monoclonal antibody to 5,6,11-trideoxytetrodotoxin, 11-nortetrodotoxin-6(S)-ol, and 11-oxotetrodotoxin, the major tetrodotoxin analogues in pufferfish

Keita Yamaki; Kyoka Sato; Yuta Kudo; Yuko Cho; Keiichi Konoki; Tomohiro Takatani; Osamu Arakawa; Kentaro Kawatsu; Mari Yotsu-Yamashita

doi:10.1016/j.toxicon.2023.107081

The quite low cross-reactivity of Kawatsu's anti-tetrodotoxin monoclonal antibody to 5,6,11-trideoxytetrodotoxin, 11-nortetrodotoxin-6(S)-ol, and 11-oxotetrodotoxin, the major tetrodotoxin analogues in pufferfish

Toxicon. 2023 Apr:226:107081. doi: 10.1016/j.toxicon.2023.107081. Epub 2023 Mar 8.

Authors

Keita Yamaki¹, Kyoka Sato¹, Yuta Kudo², Yuko Cho¹, Keiichi Konoki¹, Tomohiro Takatani³, Osamu Arakawa³, Kentaro Kawatsu⁴, Mari Yotsu-Yamashita⁵

Affiliations

¹ Graduate School of Agricultural Science, Tohoku University, 468-1 Aramaki-Aza-Aoba, Aoba-ku, Sendai, Miyagi, 980-8572, Japan.
² Graduate School of Agricultural Science, Tohoku University, 468-1 Aramaki-Aza-Aoba, Aoba-ku, Sendai, Miyagi, 980-8572, Japan; Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, 6-3 Aramaki-Aza-Aoba, Aoba-ku, Sendai, Miyagi, 980-8578, Japan.
³ Graduate School of Fisheries and Environmental Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki, Nagasaki, 852-8521, Japan.
⁴ Osaka Institute of Public Health, 1-3-3 Nakamichi, Higashinari-ku, Osaka, Osaka, 537-0025, Japan. Electronic address: kawatu@iph.osaka.jp.
⁵ Graduate School of Agricultural Science, Tohoku University, 468-1 Aramaki-Aza-Aoba, Aoba-ku, Sendai, Miyagi, 980-8572, Japan. Electronic address: mari.yamashita.c1@tohoku.ac.jp.

PMID: 36898506
DOI: 10.1016/j.toxicon.2023.107081

Abstract

The monoclonal antibody against tetrodotoxin (TTX), prepared by Kawatsu et al. (1997), has been used in several TTX-related studies. Herein, we confirmed the quite low cross-reactivity of this antibody to three major TTX analogues in pufferfish using competitive ELISA: 5,6,11-trideoxyTTX (<2.2%), 11-norTTX-6(S)-ol (<0.3%), and 11-oxoTTX (<1.5%), with reactivity against TTX being 100%. We further confirmed that the presence of these analogues did not cause a marked overestimation of TTX in pufferfish extracts using competitive ELISA.

Keywords: 11-norTetrodotoxin-6(S)-ol; 11-oxoTetrodotoxin; 5,6,11-trideoxyTetrodotoxin; ELISA; Monoclonal antibody; Tetrodotoxin.

MeSH terms

Animals
Antibodies, Monoclonal
Tetraodontiformes*

Substances

5,6,11-trideoxytetrodotoxin
11-nortetrodotoxin
11-oxotetrodotoxin
Antibodies, Monoclonal