Muscle fiber type and metabolic profiles of four muscles from the African black ostrich

Tertius A Kohn; Megan J Anley; S'thandiwe N Magwaza; Luqmaan Adamson; Louw C Hoffman; Tertius S Brand

doi:10.1016/j.meatsci.2023.109156

Muscle fiber type and metabolic profiles of four muscles from the African black ostrich

Meat Sci. 2023 Jun:200:109156. doi: 10.1016/j.meatsci.2023.109156. Epub 2023 Mar 5.

Authors

Tertius A Kohn¹, Megan J Anley², S'thandiwe N Magwaza³, Luqmaan Adamson³, Louw C Hoffman⁴, Tertius S Brand⁵

Affiliations

¹ Department of Human Biology, University of Cape Town, Private Bag X3, Rondebosch 7701, South Africa; Department of Medical Bioscience, University of the Western Cape, Private Bag X17, Bellville 7535. South Africa. Electronic address: tkohn@uwc.ac.za.
² Department of Human Biology, University of Cape Town, Private Bag X3, Rondebosch 7701, South Africa.
³ Department of Medical Bioscience, University of the Western Cape, Private Bag X17, Bellville 7535. South Africa.
⁴ Department of Animal Sciences, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa; Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation (QAAFI), Australia.
⁵ Animal Sciences, Department of Agriculture, Western Cape Government, Private Bag X1, Elsenburg 7607, South Africa; Department of Animal Sciences, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa.

PMID: 36898231
DOI: 10.1016/j.meatsci.2023.109156

Abstract

Muscle fiber type, fiber cross-sectional area (CSA), enzyme activities (citrate synthase (CS), 3-hydroxyacetyl Co A dehydrogenase (3HAD), lactate dehydrogenase (LDH) and phosphofructokinase (PFK)) and glycogen content were analyzed in the M. iliotibialis cranialis (ITC), M. iliotibialis lateralis, M. gastrocnemius (G) and M. fibularis longus (FL) muscles from 24 ostriches. Type I and II fiber proportions were similar across the 4 muscles, but the ITC had overall the smallest fibers. CS activity was the highest in the ITC, but similar between the remainder of the muscles. 3HAD activities were very low in all muscles, ranging between 1.9 and 2.7 μmol/min/g protein, indicating poor β-oxidation. The ITC also had the lowest PFK activity. Glycogen content averaged ∼85 mmol/kg dry weight across the muscles with large intramuscular variations. The 4 ostrich muscles present with low fat oxidation capacity and low glycogen content, which could have significant implications on meat quality attributes.

Keywords: Fiber type; Metabolism; Skeletal muscle.

MeSH terms

Animals
Citrate (si)-Synthase
Glycogen / metabolism
L-Lactate Dehydrogenase
Metabolome
Muscle Fibers, Skeletal / metabolism
Muscle, Skeletal / metabolism
Struthioniformes* / metabolism

Substances

L-Lactate Dehydrogenase
Citrate (si)-Synthase
Glycogen