Water-soluble protein from walleye pollock (Gadus chalcogrammus) suppresses lipopolysaccharide-induced inflammation by attenuating TLR4-MyD88 expression in macrophages

Masataka Ono; Satomi Watari; Mizuho Nishizawa-Higashi; Tatsuya Konishi; Yoshinori Takahashi; Hiroki Saeki; Ga-Hyun Joe

doi:10.1016/j.fochms.2023.100165

Water-soluble protein from walleye pollock (Gadus chalcogrammus) suppresses lipopolysaccharide-induced inflammation by attenuating TLR4-MyD88 expression in macrophages

Food Chem (Oxf). 2023 Feb 6:6:100165. doi: 10.1016/j.fochms.2023.100165. eCollection 2023 Jul 30.

Authors

Masataka Ono^{1

2}, Satomi Watari¹, Mizuho Nishizawa-Higashi², Tatsuya Konishi², Yoshinori Takahashi², Hiroki Saeki¹, Ga-Hyun Joe¹

Affiliations

¹ Faculty of Fisheries Sciences, Hokkaido University, Minato 3, Hakodate, Hokkaido 041-8611, Japan.
² Central Research Institute, Maruha Nichiro Corporation, 16-2, Wadai, Tsukuba, Ibaraki 300-4295, Japan.

Abstract

Water-soluble protein (WSP) from fish meat is abundant in the waste effluent generated via the surimi manufacturing process. This study investigated the anti-inflammatory effects and mechanisms of fish WSP using primary macrophages (MΦ) and animal ingestion. MΦ were treated with digested-WSP (d-WSP, 500 µg/mL) with or without lipopolysaccharide (LPS) stimulation. For the ingestion study, male ICR mice (5 weeks old) were fed 4% WSP for 14 days following LPS administration (4 mg/kg body weight). d-WSP decreased the expression of Tlr4, an LPS receptor. Additionally, d-WSP significantly suppressed the secretion of inflammatory cytokines, phagocytic ability, and Myd88 and Il1b expressions of LPS-stimulated macrophages. Furthermore, the ingestion of 4% WSP attenuated not only LPS-induced IL-1β secretion in the blood but also Myd88 and Il1b expressions in the liver. Thus, fish WSP decreases the expressions of the genes involved in the TLR4-MyD88 pathway in MΦ and the liver, thereby suppressing inflammation.

Keywords: 2Me, 2-mercaptoethanol; Anti-inflammation; Aq, aqua; E. coli, Escherichia coli; ELISA, Enzyme-linked immunosorbent assay; FBS, Fetal bovine serum; Fish water-soluble protein; IL-1β, Interleukin 1 beta; IL-6, Interleukin 6; LBP, Lipopolysaccharide-binding protein; LPS; LPS, Lipopolysaccharide; Macrophage; MyD88; MyD88, Myeloid differentiation primary response 88; NCDs, Noncommunicable diseases; NF-κB, Nuclear factor-kappa B; NLRP3, NACHT, LRR, and PYD domain-containing protein 3; PBS, Phosphate-buffered saline; PCR, Polymerase chain reaction; SDS, Sodium dodecyl sulfate; TICAM-1, Toll-like receptor adaptor molecule 1; TLR, Toll-like receptor; TLR4; TLR4, Toll-like receptor 4; TNF-α, Tumor necrosis factor-alpha; TNFR, Tumor necrosis factor receptor; TRIF, TIR-domain–containing adapter-inducing interferon-beta; WSP, Water-soluble protein; d-WSP, digested water-soluble protein.