A viscosity-sensitivity probe for cross-platform multimodal imaging from mitochondria to animal

Dong Wang; Zheng Gong; Wei Huang; Jun Zhao; Junlong Geng; Zhengjie Liu; Ruilong Zhang; Guangmei Han; Zhongping Zhang

doi:10.1016/j.talanta.2023.124346

A viscosity-sensitivity probe for cross-platform multimodal imaging from mitochondria to animal

Talanta. 2023 Jun 1:258:124346. doi: 10.1016/j.talanta.2023.124346. Epub 2023 Feb 11.

Authors

Dong Wang¹, Zheng Gong¹, Wei Huang¹, Jun Zhao², Junlong Geng³, Zhengjie Liu⁴, Ruilong Zhang¹, Guangmei Han⁵, Zhongping Zhang⁶

Affiliations

¹ School of Chemistry and Chemical Engineering, Information Materials and Intelligent Sensing Laboratory of Anhui Province, Key Laboratory of Structure and Functional Regulation of Hybrid Materials of Ministry of Education, Institutes of Physical Science and Information Technology, Anhui University, Hefei, Anhui, 230601, China.
² Institute of Solid-State Physics, Key Laboratory of Photovoltaic and Energy Conservation Materials, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, 230031, China.
³ School of Chemistry and Chemical Engineering, Information Materials and Intelligent Sensing Laboratory of Anhui Province, Key Laboratory of Structure and Functional Regulation of Hybrid Materials of Ministry of Education, Institutes of Physical Science and Information Technology, Anhui University, Hefei, Anhui, 230601, China. Electronic address: gengjl@ahu.edu.cn.
⁴ School of Chemistry and Chemical Engineering, Information Materials and Intelligent Sensing Laboratory of Anhui Province, Key Laboratory of Structure and Functional Regulation of Hybrid Materials of Ministry of Education, Institutes of Physical Science and Information Technology, Anhui University, Hefei, Anhui, 230601, China. Electronic address: zjliu@ahu.edu.cn.
⁵ School of Chemistry and Chemical Engineering, Information Materials and Intelligent Sensing Laboratory of Anhui Province, Key Laboratory of Structure and Functional Regulation of Hybrid Materials of Ministry of Education, Institutes of Physical Science and Information Technology, Anhui University, Hefei, Anhui, 230601, China. Electronic address: gmhan@ahu.edu.cn.
⁶ School of Chemistry and Chemical Engineering, Information Materials and Intelligent Sensing Laboratory of Anhui Province, Key Laboratory of Structure and Functional Regulation of Hybrid Materials of Ministry of Education, Institutes of Physical Science and Information Technology, Anhui University, Hefei, Anhui, 230601, China; Institute of Solid-State Physics, Key Laboratory of Photovoltaic and Energy Conservation Materials, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, 230031, China.

PMID: 36889193
DOI: 10.1016/j.talanta.2023.124346

Abstract

Viscosity in biological systems is a critical factor for various physiological process, including signal transduction and metabolisms of substance and energy. Abnormal viscosity has been proven as a key feature of many diseases, thereby real-time monitoring of viscosities in cells and in vivo is of great significance for the diagnosis and therapy of related diseases. Up to date, it is still challenging to monitor viscosity cross-platform from organelles to cells to animals with a single probe. Here, we report a benzothiazolium-xanthene probe with rotatable bonds that switch on the optical signals in high viscosity environment. The enhancements of absorption, fluorescence intensity and lifetime signals allow to dynamically monitoring the viscosity change in mitochondria and cells, while near infrared absorption and emission facilitate imaging the viscosity with both fluorescence and photoacoustic imaging in animals. The cross-platform strategy is capable of monitoring the microenvironment with multifunctional imaging across various levels.

Keywords: Cross-platform; Multimodal imaging; NIR fluorescent Probe; Viscosity.

MeSH terms

Animals
Fluorescent Dyes* / chemistry
HeLa Cells
Humans
Mitochondria / metabolism
Multimodal Imaging
Optical Imaging* / methods
Viscosity

Substances

Fluorescent Dyes