Ultrasensitive determination of α-glucosidase activity using CoOOH nanozymes and its application to inhibitor screening

Demiao Ma; Jia Ge; Ang Wang; Jingxian Li; Hongfen Yang; Wenlei Zhai; Ren Cai

doi:10.1039/d2tb02580a

Ultrasensitive determination of α-glucosidase activity using CoOOH nanozymes and its application to inhibitor screening

J Mater Chem B. 2023 Mar 22;11(12):2727-2732. doi: 10.1039/d2tb02580a.

Authors

Demiao Ma^{1

2}, Jia Ge¹, Ang Wang¹, Jingxian Li², Hongfen Yang³, Wenlei Zhai⁴, Ren Cai²

Affiliations

¹ College of Chemistry, Zhengzhou University, Zhengzhou 450001, P.R. China. jiage@zzu.edu.cn.
² Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology College of Material Science and Engineering, and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha, 410082, China. cairen@hnu.edu.cn.
³ Hunan Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, China.
⁴ Institute of Quality Standard and Testing Technology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China. zhaiwl@iqstt.cn.

PMID: 36880155
DOI: 10.1039/d2tb02580a

Abstract

In this work, a novel method for the colorimetric sensing of α-glucosidase (α-Glu) activity was developed based on CoOOH nanoflakes (NFs), which exhibit efficient oxidase-mimicking activity. Colorless 3,3',5,5'-tetramethylbenzidine (TMB) can be oxidized by CoOOH NFs into blue-colored oxidized TMB (oxTMB) in the absence of H₂O₂. L-Ascorbic acid-2-O-α-D-glucopyranose (AAG) can be hydrolysed by α-glucosidase to produce ascorbic acid, resulting in a significant decrease of catalytic activity of CoOOH NFs. Thus, a colorimetric α-glucosidase activity detection method was designed with a limit of detection of 0.0048 U mL^-1. Furthermore, the designed sensing platform exhibits favorable applicability for the α-glucosidase (α-Glu) activity assay in real samples. Meanwhile, this method can be expanded to study the inhibitors of α-Glu. Finally, the as-proposed method combined with a smartphone would be a color recognizer, which was successfully applied for the determination of α-Glu activity in human serum samples.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Humans
Hydrogen Peroxide*
Oxides
Oxidoreductases
alpha-Glucosidases*

Substances

HCoO2
alpha-Glucosidases
Hydrogen Peroxide
Oxides
Oxidoreductases