MiRNA-23a-5p is the biomarkers for gouty arthritis and promotes inflammation in rats of gouty arthritis via MyD88/NF-κB pathway by induction TLR2

Fang Li; Jian-Hua Yao; Li Li; Qian Nie; Jing-Jing Cao; Xiao-Ran Ning

doi:10.46497/ArchRheumatol.2022.9236

MiRNA-23a-5p is the biomarkers for gouty arthritis and promotes inflammation in rats of gouty arthritis via MyD88/NF-κB pathway by induction TLR2

Arch Rheumatol. 2022 Jul 29;37(4):536-546. doi: 10.46497/ArchRheumatol.2022.9236. eCollection 2022 Dec.

Authors

Fang Li¹, Jian-Hua Yao², Li Li³, Qian Nie⁴, Jing-Jing Cao¹, Xiao-Ran Ning¹

Affiliations

¹ Department of Rheumatism and Immunology, Hebei General Hospital, Shijiazhuang, China.
² Department of Geratology, The Second Hospital of Hebei Medical University, Shijiazhuang, China.
³ Department Ultrasonograph, Hebei General Hospital, Shijiazhuang, China.
⁴ Hebei General Hospital, Medicai Examination Center, Shijiazhuang, China.

Abstract

Objectives: In this study, we aimed to examine the efficacy of micro ribonucleic acid (miRNA)-23a-5p in gouty arthritis and to investigate its possible mechanism.

Materials and methods: Gouty arthritis in rat was established by intraarticular injection of 0.2 mL monosodium urate crystal (20 mg/mL) inside knee joint cavity. THP-1 cell was induced using lipopolysaccharides (LPS) for in vitro model.

Results: Serum miRNA-23a-5p expression levels were increased in rats of gouty arthritis. However, overexpression of miRNA-23a-5p promoted inflammation and induced myeloid differential protein-88 (MyD88)/nuclear factor-kappa B (NF-κB) pathway by induction toll-like receptor-2 (TLR2) in vitro. The inhibition of TLR2 attenuated the pro-inflammation effects of miRNA-23a-5p in inflammation in in vitro model of gouty arthritis.

Conclusion: Our findings demonstrate that miRNA-23a-5p is a biomarker for gouty arthritis and promotes inflammation in rats of gouty arthritis via MyD88/NF-κB pathway by targeting TLR2.

Keywords: Gouty arthritis; MyD88; NF-κB; TLR2.; inflammation; miRNA-23a-5p.