A cyanobiphenyl-based fluorescent ''lighting-up'' sensor for highly selective and sensitive recognition of Al3+: Theoretical, practical and bioimaging studies

Ozlem Guctekin Yasar; Aykut Bostanci; Sukriye Nihan Karuk Elmas; Duygu Aydin; Fatma Nur Arslan; Gokhan Sadi; Ibrahim Yilmaz

doi:10.1016/j.saa.2023.122556

A cyanobiphenyl-based fluorescent ''lighting-up'' sensor for highly selective and sensitive recognition of Al³⁺: Theoretical, practical and bioimaging studies

Spectrochim Acta A Mol Biomol Spectrosc. 2023 Jun 5:294:122556. doi: 10.1016/j.saa.2023.122556. Epub 2023 Feb 28.

Authors

Ozlem Guctekin Yasar¹, Aykut Bostanci², Sukriye Nihan Karuk Elmas³, Duygu Aydin⁴, Fatma Nur Arslan⁵, Gokhan Sadi², Ibrahim Yilmaz¹

Affiliations

¹ Chemistry Department, Kamil Ozdag Science Faculty, Karamanoglu Mehmetbey University, 70100 Karaman, Turkiye.
² Biology Department, Kamil Ozdag Science Faculty, Karamanoglu Mehmetbey University, 70100 Karaman, Turkiye.
³ Chemistry Department, Kamil Ozdag Science Faculty, Karamanoglu Mehmetbey University, 70100 Karaman, Turkiye; Analytical Chemistry Department, Pharmacy Faculty, Istanbul University-Cerrahpaşa, 34500 Istanbul, Turkiye. Electronic address: snihankaruk@gmail.com.
⁴ Chemistry Department, Kamil Ozdag Science Faculty, Karamanoglu Mehmetbey University, 70100 Karaman, Turkiye. Electronic address: duyguaydin@kmu.edu.tr.
⁵ Chemistry Department, Kamil Ozdag Science Faculty, Karamanoglu Mehmetbey University, 70100 Karaman, Turkiye. Electronic address: arslanfatmanur@gmail.com.

PMID: 36878135
DOI: 10.1016/j.saa.2023.122556

Abstract

The recognition of toxic Al³⁺ in foods and biosystems has of great interest to researchers. Herein, a novel cyanobiphenyl-based chemosensor CATH (E)-N'-((4'-cyano-4-hydroxy-[1,1'-biphenyl]-3-yl)methylene)thiophene-2-carbohydrazide was fabricated and shown to recognize Al³⁺ in HEPES buffer:EtOH (90:10, v:v, pH = 7.4) by ''lighting-up'' fluorescence sensing. The CATH evidenced high sensitivity (LOD = 13.1 nM) and excellent selectivity to Al³⁺ over competing cations. The Job's plot, TOF-MS and theoretical computation studies were performed to probe the binding mechanism of Al³⁺ to CATH. Additionally; CATH was successfully utilized to practical applications and employed to recover of Al³⁺ from different food samples. More importantly, it was employed to intracellular Al³⁺ detection in living cells including THLE2 and HepG2.

Keywords: Aluminum; DFT; Fluorescent sensor; Food; Living cell.

MeSH terms

Aluminum*
Cations
Fluorescence
Fluorescent Dyes* / metabolism
Spectrometry, Fluorescence / methods

Substances

Aluminum
Fluorescent Dyes
Cations