Slc9a1 plays a vital role in chitosan oligosaccharide transport across the intestinal mucosa of mice

Jiaying Wen; Shengwei Chen; Minglong Bao; Canying Hu; Lianyun Wu; Yanhong Yong; Xiaoxi Liu; Youquan Li; Zhichao Yu; Xingbin Ma; Jong-Bang Eun; Jae-Han Shim; Mohamad Warda; A M Abd El-Aty; Xianghong Ju

doi:10.1016/j.carbpol.2022.120179

Slc9a1 plays a vital role in chitosan oligosaccharide transport across the intestinal mucosa of mice

Carbohydr Polym. 2023 Jan 1:299:120179. doi: 10.1016/j.carbpol.2022.120179. Epub 2022 Sep 30.

Authors

Jiaying Wen¹, Shengwei Chen¹, Minglong Bao¹, Canying Hu², Lianyun Wu², Yanhong Yong¹, Xiaoxi Liu³, Youquan Li³, Zhichao Yu³, Xingbin Ma³, Jong-Bang Eun⁴, Jae-Han Shim⁵, Mohamad Warda⁶, A M Abd El-Aty⁷, Xianghong Ju⁸

Affiliations

¹ Marine Medical Research and Development Centre, Shenzhen Institute of Guangdong Ocean University, Shenzhen 518120, China; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China; The Key Laboratory of Animal Resources and Breed Innovation in Western Guangdong Province, Zhanjiang 524088, China.
² Marine Medical Research and Development Centre, Shenzhen Institute of Guangdong Ocean University, Shenzhen 518120, China; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.
³ College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China; The Key Laboratory of Animal Resources and Breed Innovation in Western Guangdong Province, Zhanjiang 524088, China.
⁴ Department of Food Science and Technology, Chonnam National University, Gwangju, Republic of Korea.
⁵ Natural Products Chemistry Laboratory, Biotechnology Research Institute, Chonnam National University, Yongbong-ro, Buk-gu, Gwangju 500-757, Republic of Korea.
⁶ Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.
⁷ State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China; Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt; Department of Medical Pharmacology, Medical Faculty, Ataturk University, Erzurum 25240, Turkey. Electronic address: abdelaty44@hotmail.com.
⁸ Marine Medical Research and Development Centre, Shenzhen Institute of Guangdong Ocean University, Shenzhen 518120, China; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China; The Key Laboratory of Animal Resources and Breed Innovation in Western Guangdong Province, Zhanjiang 524088, China. Electronic address: juxh77@163.com.

PMID: 36876794
DOI: 10.1016/j.carbpol.2022.120179

Abstract

The mechanism underlying the intestinal transport of COS is not well understood. Here, transcriptome and proteome analyses were performed to identify potential critical molecules involved in COS transport. Enrichment analyses revealed that the differentially expressed genes in the duodenum of the COS-treated mice were mainly enriched in transmembrane and immune function. In particular, B2 m, Itgb2, and Slc9a1 were upregulated. The Slc9a1 inhibitor decreased the transport efficiency of COS both in MODE-K cells (in vitro) and in mice (in vivo). The transport of FITC-COS in Slc9a1-overexpressing MODE-K cells was significantly higher than that in empty vector-transfected cells (P < 0.01). Molecular docking analysis revealed the possibility of stable binding between COS and Slc9a1 through hydrogen bonding. This finding indicates that Slc9a1 plays a crucial role in COS transport in mice. This provides valuable insights for improving the absorption efficiency of COS as a drug adjuvant.

Keywords: Chitosan oligosaccharide; Intestine; Mice; Transcriptome; Transport molecule.

MeSH terms

Animals
Biological Transport*
Chitosan*
Intestinal Mucosa* / metabolism
Mice
Molecular Docking Simulation
Oligosaccharides
Sodium-Hydrogen Exchanger 1* / metabolism

Substances

Chitosan
Oligosaccharides
Slc9a1 protein, mouse
Sodium-Hydrogen Exchanger 1