Anti-inflammatory constituents isolated from the flowers of Hosta plantaginea via suppression of the NF-κB signaling pathway in LPS-stimulated RAW 264.7 macrophages

Jie-Wei He; Ping Guo; Li Yang; Jun-Wei He

doi:10.1039/d2ra07623c

Anti-inflammatory constituents isolated from the flowers of Hosta plantaginea via suppression of the NF-κB signaling pathway in LPS-stimulated RAW 264.7 macrophages

RSC Adv. 2023 Mar 3;13(11):7179-7184. doi: 10.1039/d2ra07623c. eCollection 2023 Mar 1.

Authors

Jie-Wei He¹, Ping Guo², Li Yang¹, Jun-Wei He¹

Affiliations

¹ Jiangxi University of Chinese Medicine Nanchang 330004 China hjwjn2008@163.com.
² Department of Medical Laboratory, First Affiliated Hospital of Nanchang University Nanchang 330006 China.

Abstract

Hosta plantaginea (Lam.) Aschers flower is traditionally used in China as an important herbal medicine for the treatment of inflammatory disease. The present study isolated one new compound, namely (3R)-dihydrobonducellin (1), and five known ones, p-hydroxycinnamic acid (2), paprazine (3), thymidine (4), bis(2-ethylhexyl) phthalate (5), and dibutyl phthalate (6) from H. plantaginea flowers. These structures were elucidated from spectroscopic data. Among them, compounds 1-4 remarkably suppressed nitric oxide (NO) production in lipopolysaccharide (LPS)-induced RAW 264.7 cells with half maximal inhibitory concentration (IC₅₀) values of 19.88 ± 1.81, 39.80 ± 0.85, 19.03 ± 2.35, and 34.63 ± 2.38 μM, respectively. Furthermore, compounds 1 and 3 (20 μM) significantly decreased levels of tumor necrosis factor α (TNF-α), prostaglandin E2 (PGE2), interleukin 1β (IL-1β), and IL-6. Additionally, compounds 1 and 3 (20 μM) prominently reduced the phosphorylation protein level of nuclear factor kappa-B (NF-κB) p65. The present findings indicated that compounds 1 and 3 may be new candidates against inflammation via blocking the NF-κB signaling pathway.