Jaboticaba (Myrciaria jaboticaba) peel extracts induce reticulum stress and apoptosis in breast cancer cells

Juliana Kelly da Silva-Maia; Arumugam Nagalingam; Cinthia Baú Betim Cazarin; Mário Roberto Marostica Junior; Dipali Sharma

doi:10.1016/j.fochms.2023.100167

Jaboticaba (Myrciaria jaboticaba) peel extracts induce reticulum stress and apoptosis in breast cancer cells

Food Chem (Oxf). 2023 Feb 22:6:100167. doi: 10.1016/j.fochms.2023.100167. eCollection 2023 Jul 30.

Authors

Juliana Kelly da Silva-Maia^{1

2

3}, Arumugam Nagalingam², Cinthia Baú Betim Cazarin³, Mário Roberto Marostica Junior³, Dipali Sharma²

Affiliations

¹ Nutrition Postgraduate Program, Department of Nutrition, Health Science Center, Federal University of Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte, Brazil.
² Department of Oncology, Johns Hopkins University School of Medicine and Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD, United States.
³ Department of Food and Nutrition, School of Food Engineering, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil.

Abstract

Jaboticaba peel (Myrciaria jaboticaba) is a source of bioactive compounds. We investigated the anticancer activity of ethyl acetate extract (JE1) and hydroethanolic extract (JE2) of Jaboticaba peel against breast cancer. Both JE1 and JE2 inhibited clonogenic potential of MDA-MB-231 cells while JE1 was particularly effective in MCF7 cells. Anchorage-independent growth and cell viability was also inhibited by JE1 and JE2. In addition to growth inhibition, JE1 and JE2 could also inhibit migration and invasion of cells. Interestingly, JE1 and JE2 show selective inhibition towards certain breast cancer cells and biological processes. Mechanistic evaluations showed that JE1 induced PARP cleavage, BAX and BIP indicating apoptotic induction. An elevation of phosphorylated ERK was observed in MCF7 cells in response to JE1 and JE2 along with increased IRE-α and CHOP expression indicating increased endoplasmic stress. Therefore, Jaboticaba peel extracts could be potentially considered for further development for breast cancer inhibition.

Keywords: Antitumor; Bioactive compounds; Breast cancer; Myrciaria jaboticaba.