Synergetic effect of water deficit and arbuscular mycorrhizal symbiosis on the expression of aquaporins in wheat (Triticum aestivum L.) roots: insights from NGS RNA-sequencing

Maryam Asadollahi; Alireza Iranbakhsh; Rahim Ahmadvand; Mostafa Ebadi; Iraj Mehregan

doi:10.1007/s12298-023-01285-w

Synergetic effect of water deficit and arbuscular mycorrhizal symbiosis on the expression of aquaporins in wheat (Triticum aestivum L.) roots: insights from NGS RNA-sequencing

Physiol Mol Biol Plants. 2023 Feb;29(2):195-208. doi: 10.1007/s12298-023-01285-w. Epub 2023 Feb 6.

Authors

Maryam Asadollahi¹, Alireza Iranbakhsh¹, Rahim Ahmadvand², Mostafa Ebadi³, Iraj Mehregan¹

Affiliations

¹ Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
² Department of Vegetables Research, Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran.
³ Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran.

Abstract

Wheat (Triticum aestivum) is one of the most important crops in the world. This investigation was attempted to evaluate the transcriptional responses of aquaporins (AQPs) to the mycorrhizal inoculation and/or water deficit conditions in wheat to clarify how the arbuscular mycorrhizal symbiosis can contribute to the modulation of water homeostasis. The wheat seedlings were subjected to the water deficiency, and mycorrhizal inoculation using arbuscular fungus Funneliformis mosseae and Illumina RNA-Seq analyses confirmed that aquaporins expressed differentially in response to both the irrigation levels and mycorrhizal colonization. Results of this study showed that only 13% of the studied AQPs were responsive to water deficit with a tiny fraction (3%) being up-regulated. Mycorrhizal inoculation had a greater impact on the expression of AQPs with ca. 26% being responsive, ca. 4% of which were up-regulated. The samples with arbuscular mycorrhizal inoculation yielded more root and stem biomass. Water deficit and mycorrhizal inoculation caused different AQPs to be up-regulated. The effect of mycorrhizal inoculation on the expression of AQPs was intensified by applying water deficiency with 32% of studied AQPs being responsive, 6% of which up-regulated. We also found that the overexpression of three genes TaNIP1-10, TaNIP3-3, and TaNIP3-4 was chiefly triggered by mycorrhizal inoculation. Our results show that water deficit has a lower impact on the expression of aquaporins compared to what the arbuscular mycorrhizal inoculation has; water deficit and arbuscular mycorrhizal inoculation mainly cause the down-regulation of the aquaporins, and water deficit and the arbuscular inoculation have synergetic effects. These findings could improve our knowledge of how arbuscular mycorrhizal symbiosis can contribute to the modulation of water homeostasis.

Supplementary information: The online version contains supplementary material available at 10.1007/s12298-023-01285-w.

Keywords: Aquaporins; Mycorrhizal symbiosis; NGS; Transcriptome; Water deficit.

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