MbMYBC1, a M. baccata MYB transcription factor, contribute to cold and drought stress tolerance in transgenic Arabidopsis

Wanda Liu; Tianhe Wang; Yu Wang; Xiaoqi Liang; Jilong Han; Deguo Han

doi:10.3389/fpls.2023.1141446

MbMYBC1, a M. baccata MYB transcription factor, contribute to cold and drought stress tolerance in transgenic Arabidopsis

Front Plant Sci. 2023 Feb 16:14:1141446. doi: 10.3389/fpls.2023.1141446. eCollection 2023.

Authors

Wanda Liu¹, Tianhe Wang¹, Yu Wang¹, Xiaoqi Liang², Jilong Han¹, Deguo Han²

Affiliations

¹ Horticulture Branch, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang, China.
² Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs/National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold Regions/College of Horticulture & Landscape Architecture, Northeast Agricultural University, Harbin, China.

Abstract

Cold and drought stress considerably suppress the development of plants. In this study, a new MYB (v-myb avian myeloblastosis viral)TF gene, MbMYBC1, was isolated from the M. baccata and located in nucleus. MbMYBC1 has a positive response to low temperature and drought stress. After being introduced into Arabidopsis thaliana, the physiological indicators of transgenic Arabidopsis had corresponding changes under these two stresses, the activities of catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD) increased, electrolyte leakage rate (EL) and the content of proline increased, but the content of chlorophyll decreased. In addition, its overexpression can also activate the downstream expression of AtDREB1A, AtCOR15a, AtERD10B and AtCOR47 related to cold stress and AtSnRK2.4, AtRD29A, AtSOD1and AtP5CS1 related to drought stress. Based on these results, we speculate that MbMYBC1 can respond to cold and hydropenia signals, and can be used in transgenic technology to improve plant tolerance to low temperature and drought stress.

Keywords: Malus baccata (L.) Borkh; MbMYBC1; cold stress; drought stress; transgenic technology.

Grants and funding

This project was supported by the National Natural Science Foundation of China (32172521), the Natural Science Fund Joint Guidance Project of Heilongjiang Province (LH2022C097), the Collaborative Innovation System of Agricultural Bioeconomy in Heilongjiang Province and Heilongjiang Province Research Institute Research Expenses Project (CZKYF2023-1-B021).