Optical coherence microscopy with a split-spectrum image reconstruction method for temporal-dynamics contrast-based imaging of intracellular motility

Yong Guk Kang; Raymart Jay E Canoy; Yongjun Jang; Ana Rita M P Santos; Inwoo Son; Beop-Min Kim; Yongdoo Park

doi:10.1364/BOE.478264

Optical coherence microscopy with a split-spectrum image reconstruction method for temporal-dynamics contrast-based imaging of intracellular motility

Biomed Opt Express. 2023 Jan 4;14(2):577-592. doi: 10.1364/BOE.478264. eCollection 2023 Feb 1.

Authors

Yong Guk Kang^{1

2}, Raymart Jay E Canoy^{3

2}, Yongjun Jang⁴, Ana Rita M P Santos⁴, Inwoo Son⁴, Beop-Min Kim^{1

5

6}, Yongdoo Park⁴

Affiliations

¹ BK21 Four Institute of Precision Public Health, Korea University, Seoul 02841, Republic of Korea.
² These authors contributed equally to this work.
³ Department of Biomicro System Technology, College of Engineering, Korea University, Seoul 02841, Republic of Korea.
⁴ Department of Biomedical Science, College of Medicine, Korea University, Seoul 02841, Republic of Korea.
⁵ Department of Biomedical Engineering, College of Health Science, Korea University, Seoul 02841, Republic of Korea.
⁶ bmk515@korea.ac.kr.

Abstract

Biomedical researchers use optical coherence microscopy (OCM) for its high resolution in real-time label-free tomographic imaging. However, OCM lacks bioactivity-related functional contrast. We developed an OCM system that can measure changes in intracellular motility (indicating cellular process states) via pixel-wise calculations of intensity fluctuations from metabolic activity of intracellular components. To reduce image noise, the source spectrum is split into five using Gaussian windows with 50% of the full bandwidth. The technique verified that F-actin fiber inhibition by Y-27632 reduces intracellular motility. This finding could be used to search for other intracellular-motility-associated therapeutic strategies for cardiovascular diseases.