Evaluation of the antibacterial activity of Weissella confusa K3 cell-free supernatant against extended-spectrum βeta lactamase (ESBL) producing uropathogenic Escherichia coli U60

Rasha M El-Mekkawy; Noura E Hamour; Wesam A Hassanein; Ayman A Allam

doi:10.1016/j.sjbs.2023.103595

Evaluation of the antibacterial activity of Weissella confusa K3 cell-free supernatant against extended-spectrum βeta lactamase (ESBL) producing uropathogenic Escherichia coli U60

Saudi J Biol Sci. 2023 Apr;30(4):103595. doi: 10.1016/j.sjbs.2023.103595. Epub 2023 Feb 21.

Authors

Rasha M El-Mekkawy¹, Noura E Hamour¹, Wesam A Hassanein¹, Ayman A Allam²

Affiliations

¹ Department of Botany and Microbiology, Faculty of Science, Zagazig University, 44519, Egypt.
² Department of Medical Microbiology and Immunology, Faculty of Medicine, Zagazig University, Egypt.

Abstract

Different strategies have been approved for controlling extended-spectrum βeta lactamase (ESBL) producing uropathogenic bacteria. The antibacterial activity of Lactic acid bacteria (LAB) is an effective strategy due to its probiotic characteristics and beneficial effects on human health. The antibiotic susceptibility test, disk diffusion method, and double disc synergy test indicated that five enteric uropathogenic isolates were ESBL producers during the present study. They recorded diameters of inhibition zones as ≤ 18, ≤ 8, ≤ 19, and ≤ 8 mm against cefotaxime (CTX), ceftazidime (CAZ), aztreonam (ATM), and ceftriaxone (CRO). Genotypically, bla_TEM genes are the most common, with (100 %) occurrence in all the five enteric tested uropathogens, followed by bla_SHV and bla_CTX genes (60 %). In addition, out of 10 LAB isolates from dairy products, the CFS of isolate no. K3 had high antibacterial activity against the tested ESBLs, especially no. U60, with a MIC of 600 µl. Additionally, the MIC and sub-MIC of K3 CFS inhibited the production of antibiotic-resistant bla _TEM genes of U60. Analyzing the 16S rRNA sequence confirmed that the most potent ESBL-producing bacteria (U60) and LAB (K3) isolates were identified as Escherichia coli U60.1 and Weissella confuse K3 with accession numbers MW173246 and MW173299.1, respectively, in GenBank.

Keywords: Antibacterial activity; Antibiotic resistance; ECU60, Escherichia coli U60; ESBL, extended-spectrum beta-lactamase; Extended-spectrum beta-lactamase; LAB, lactic acid bacteria; Lactic acid bacteria; MHA, Mueller–Hinton agar; MRD, Multidrug resistance; MRS, De Man, Rogosa, and Sharpe; U, Urine sample; UTI, Urinary tract infection; Urinary tract infection; WC K3, Weissella confusa K3.

Publication types

Review