AdipoRon accelerates bone repair of calvarial defect in diet-induced obesity mice

Xingwen Wu; Danting Zhu; Le Shi; Qisheng Tu; Youcheng Yu; Jake Chen

doi:10.1016/j.heliyon.2023.e13975

AdipoRon accelerates bone repair of calvarial defect in diet-induced obesity mice

Heliyon. 2023 Feb 24;9(3):e13975. doi: 10.1016/j.heliyon.2023.e13975. eCollection 2023 Mar.

Authors

Xingwen Wu^{1

2}, Danting Zhu³, Le Shi³, Qisheng Tu², Youcheng Yu¹, Jake Chen²

Affiliations

¹ Dept. of Dentistry, Zhongshan Hospital, Fudan University, Shanghai, China.
² Division of Oral Biology, Tufts University School of Dental Medicine, Boston, USA.
³ Department of Stomatology, Dental Center of Jing-An District, Shanghai, PR China.

Abstract

Objectives: To investigate the role of AdipoRon in bone wound healing of calvaria critical-sized defects (CSD) in diet-induced obesity (DIO) mice.

Materials and methods: After establishing the calvaria CSD in normal-chow (NC), DIO and Adiponectin knockout (APNKO) mice, AdipoRon or vehicle was orally gavaged for 3 weeks. The bone defects were analyzed by micro-CT and H&E staining. The expression of osteogenesis-related factor in the defect area, and the chemotactic gradient of SDF-1 between bone marrow and bone defect area were further analyzed.

Results: AdipoRon downregulated body weight and alleviated fasting blood glucose level of DIO mice after treatment with AdipoRon in 14 and 21 days. Newly formed bone was significantly increased in the defect area of DIO and APNKO mice after treatment with AdipoRon compared with vehicle treatment. No significant difference was shown in NC mice. Furthermore, compared with NC mice, a significant decrease of BV/TV%, Tb.N value and formed bone percentage were shown in DIO and APNKO mice. The treatment with AdipoRon could reverse of decreased value and increase the newly formed bone in those mice. AdipoRon promoted col-1α expression in wound sites in DIO and APNKO mice. AdipoRon nearly quadrupled the chemotactic gradient of SDF-1 by decreasing SDF-1 expression in bone marrow and increasing it in the bone defect area in APNKO and DIO treated mice.

Conclusion: AdipoRon alleviates the obesity status in DIO mice with calvarial defect and increase new bone formation in calvarial defects in DIO and APNKO mice by modulating chemotactic gradient of SDF-1.

Keywords: AdipoRon; Adiponectin; Adiponectin knockout mice; Bone wound healing; Calvaria critical-sized defects; Diet-induced obesity mice.