The intervention effects of konjac glucomannan with different molecular weights on high-fat and high-fructose diet-fed obese mice based on the regulation of gut microbiota

Qian Liu; Jie Fang; Wenqi Huang; Sining Liu; Xueting Zhang; Guiping Gong; Linjuan Huang; Xiaoliang Lin; Zhongfu Wang

doi:10.1016/j.foodres.2023.112498

The intervention effects of konjac glucomannan with different molecular weights on high-fat and high-fructose diet-fed obese mice based on the regulation of gut microbiota

Food Res Int. 2023 Mar:165:112498. doi: 10.1016/j.foodres.2023.112498. Epub 2023 Jan 24.

Authors

Qian Liu¹, Jie Fang², Wenqi Huang¹, Sining Liu¹, Xueting Zhang¹, Guiping Gong¹, Linjuan Huang¹, Xiaoliang Lin³, Zhongfu Wang⁴

Affiliations

¹ Shaanxi Natural Carbohydrate Resource Engineering Research Center, College of Food Science and Technology, Northwest University, Xi'an 710069, China.
² College of Life Sciences, Northwest University, Xi'an 710069, China.
³ Infinitus (China) Company Ltd., Guangzhou 510000, Guangdong, China. Electronic address: Ivan.Lin@infinitus-int.com.
⁴ Shaanxi Natural Carbohydrate Resource Engineering Research Center, College of Food Science and Technology, Northwest University, Xi'an 710069, China. Electronic address: wangzhf@nwu.edu.cn.

PMID: 36869507
DOI: 10.1016/j.foodres.2023.112498

Abstract

Konjac is a high-quality dietary fiber rich in β-glucomannan, which has been reported to possess anti-obesity effects. To explore the effective components and the structure-activity relationships of konjac glucomannan (KGM), three different molecular weight components (KGM-1 (90 kDa), KGM-2 (5 kDa), KGM-3 (1 kDa)) were obtained, and systematical comparisons of their effects on high-fat and high-fructose diet (HFFD)-induced obese mice were investigated in the present study. Our results indicated that KGM-1, with its larger molecular weight, reduced mouse body weight and improved their insulin resistance status. KGM-1 markedly inhibited lipid accumulation in mouse livers induced by HFFD by downregulating Pparg expression and upregulating Hsl and Cpt1 expressions. Further investigation revealed that dietary supplementation with konjac glucomannan at different molecular weights caused β-diversity changes in gut microbes. The potential weight loss effect of KGM-1 maybe attributed to the abundance of changes in Coprobacter, Streptococcus, Clostridium IV, and Parasutterella. The results provide a scientific basis for the in-depth development and utilization of konjac resources.

Keywords: Gut microbiota; High–calorie diet; Konjac glucomannan; Obesity; Structure–activity relationships.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amorphophallus*
Animals
Diet
Fructose
Gastrointestinal Microbiome*
Mice
Mice, Obese
Molecular Weight

Substances

(1-6)-alpha-glucomannan
Fructose