Immunohistochemical localization of glucocorticoid receptors in the human cochlea

Hirooki Matsui; Ivan A Lopez; Gail Ishiyama; Akira Ishiyama

doi:10.1016/j.brainres.2023.148301

Immunohistochemical localization of glucocorticoid receptors in the human cochlea

Brain Res. 2023 May 1:1806:148301. doi: 10.1016/j.brainres.2023.148301. Epub 2023 Mar 1.

Authors

Hirooki Matsui¹, Ivan A Lopez², Gail Ishiyama³, Akira Ishiyama⁴

Affiliations

¹ Department of Head and Neck Surgery, David Geffen School of Medicine at UCLA, University of California, Los Angeles, USA; Department of Otolaryngology, Head and Neck Surgery, Yamagata University School of Medicine, Yamagata, Japan.
² Department of Head and Neck Surgery, David Geffen School of Medicine at UCLA, University of California, Los Angeles, USA. Electronic address: ilopez@ucla.edu.
³ Department of Neurology. David Geffen School of Medicine at UCLA, University of California, Los Angeles, USA.
⁴ Department of Head and Neck Surgery, David Geffen School of Medicine at UCLA, University of California, Los Angeles, USA.

PMID: 36868509
PMCID: PMC10521330 (available on 2024-05-01)
DOI: 10.1016/j.brainres.2023.148301

Abstract

In the present study we investigated the localization of glucocorticoid receptors (GCR) in the human inner ear using immunohistochemistry. Celloidin-embedded cochlear sections of patients with normal hearing (n = 5), patients diagnosed with MD (n = 5), and noise induced hearing loss (n = 5) were immunostained using GCR rabbit affinity-purified polyclonal antibodies and secondary fluorescent or HRP labeled antibodies. Digital fluorescent images were acquired using a light sheet laser confocal microscope. In celloidin-embedded sections GCR-IF was present in the cell nuclei of hair cells and supporting cells of the organ of Corti. GCR-IF was detected in cell nuclei of the Reisner's membrane. GCR-IF was seen in cell nuclei of the stria vascularis and the spiral ligament. GCR-IF was found in the spiral ganglia cell nuclei, however, spiral ganglia neurons showed no GCR-IF. Although GCRs were found in most cell nuclei of the cochlea, the intensity of IF was differential among the different cell types being more intense in supporting cells than in sensory hair cells. The differential expression of GCR receptors found in the human cochlea may help to understand the site of action of glucocorticoids in different ear diseases.

Keywords: Celloidin-embedded sections; Cochlea; Glucocorticoid receptors; Human inner ear.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Animals
Cochlea / metabolism
Collodion / metabolism
Ear, Inner* / metabolism
Humans
Rabbits
Receptors, Glucocorticoid* / metabolism
Spiral Ganglion / metabolism

Substances

Receptors, Glucocorticoid
Collodion

Abstract

Publication types

MeSH terms

Substances

Grants and funding