Evaluation of expression of biomarkers of PLAGL1 (ZAC1), microRNA, and their non-coding RNAs in patients with endometriosis

J Gynecol Obstet Hum Reprod. 2023 Apr;52(4):102568. doi: 10.1016/j.jogoh.2023.102568. Epub 2023 Mar 2.

Abstract

Since the PLAGL1 (ZAC1) gene is expressed in the human endometrium. It may be involved in the etiology of endometrial disorders by its abnormal regulation and expression. This study aimed to investigate the Zac1 gene and related microRNA and LncRNA and its alterations in patients with endometriosis. Blood plasma, ectopic (EC) and eutopic (EU) endometrial samples were gathered from 30 patients with endometriosis and 30 healthy fertile women, and the Q-PCR technique was used to determine the expression level of Zac1 mRNA and microRNAs (miR-1271-5p, hsa-miR-490-3pin) and LncRNAs (TONSL-AS1 TONSL, KCNQ1OT1 KCNQ1). According to the results, the Zac1 gene and KCNQ1OT1 KCNQ1, TONSL-AS1 TONSL LncRNA expression were significantly decreased in the endometriosis group versus the control group (P < 0.05). MiR-1271-5p and hsa-miR-490-3pin microRNA expression were significantly raised in the endometriosis group as opposed to the control group (P < 0.05). In summary, this research for the first time revealed that identifying Zac1 expression provides us with new indicators for evaluating endometriosis.

Keywords: Biomarker; Endometriosis; LncRNAs; MicroRNA; PLAGL1 (Zac1).

MeSH terms

  • Biomarkers
  • Cell Cycle Proteins
  • Endometriosis* / genetics
  • Endometriosis* / metabolism
  • Female
  • Humans
  • KCNQ1 Potassium Channel
  • MicroRNAs* / genetics
  • NF-kappa B / metabolism
  • RNA, Long Noncoding* / genetics
  • RNA, Long Noncoding* / metabolism
  • Transcription Factors
  • Tumor Suppressor Proteins / metabolism

Substances

  • RNA, Long Noncoding
  • KCNQ1 Potassium Channel
  • MicroRNAs
  • Biomarkers
  • PLAGL1 protein, human
  • Transcription Factors
  • Cell Cycle Proteins
  • Tumor Suppressor Proteins
  • TONSL protein, human
  • NF-kappa B
  • MIRN1271 microRNA, human