Anti-inflammatory effects of Derris scandens extract on narrowband-ultraviolet B exposed HaCaT human keratinocytes

Sumrit Sukhonthasilakun; Pramote Mahakunakorn; Alisa Naladta; Katesaraporn Nuankaew; Somsak Nualkaew; Chavi Yenjai; Natsajee Nualkaew

doi:10.1016/j.jaim.2023.100693

Anti-inflammatory effects of Derris scandens extract on narrowband-ultraviolet B exposed HaCaT human keratinocytes

J Ayurveda Integr Med. 2023 Mar-Apr;14(2):100693. doi: 10.1016/j.jaim.2023.100693. Epub 2023 Mar 1.

Authors

Sumrit Sukhonthasilakun¹, Pramote Mahakunakorn¹, Alisa Naladta², Katesaraporn Nuankaew¹, Somsak Nualkaew³, Chavi Yenjai⁴, Natsajee Nualkaew⁵

Affiliations

¹ Division of Pharmacognosy and Toxicology, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
² Department of Biochemistry, Faculty of Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
³ Pharmaceutical Chemistry and Natural Product Research Unit, Faculty of Pharmacy, Mahasarakham University, Mahasarakham, 44150, Thailand.
⁴ Natural Products Research Unit, Center of Excellence for Innovation in Chemistry, Department of Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand.
⁵ Division of Pharmacognosy and Toxicology, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand. Electronic address: nnatsa@kku.ac.th.

Abstract

Narrowband-ultraviolet B (NB-UVB) has been used to treat skin diseases such as psoriasis. Chronic use of NB-UVB might cause skin inflammation and lead to skin cancer. In Thailand, Derris Scandens (Roxb.) Benth. is used as an alternative medicine to nonsteroidal anti-inflammatory drugs (NSAIDs) for low back pain and osteoarthritis. Therefore, this study aimed to evaluate the potential anti-inflammatory effect of Derris scandens extract (DSE) on pre- and post exposed NB-UVB human keratinocytes (HaCaT). The results indicated that DSE could not protect HaCaT from cell morphology changes or DNA fragmentation and could not recover cell proliferation ability from the NB-UVB effects. DSE treatment reduced the expression of genes related to inflammation, collagen degradation, and carcinogenesis, such as IL-1α, IL-1β, IL-6, iNOS, COX-2, MMP-1, MMP-9, and Bax. These results indicated the potential use of DSE as a topical preparation against NB-UVB-induced inflammation, anti-aging, and prevention of skin cancer from phototherapy.

Keywords: Anti-inflammation; Derris scandens; Human keratinocytes; Narrowband-ultraviolet B; Phototherapy; UV protection.