Au@SiO2 SERS nanotags based lateral flow immunoassay for simultaneous detection of aflatoxin B1 and ochratoxin A

Ruipeng Chen; Hui Wang; Chaoqun Sun; Yiguang Zhao; Yue He; Muhammad Shemyal Nisar; Wensong Wei; Haiqi Kang; Xiulan Xie; Chunmei Du; Qingyao Luo; Liang Yang; Xiangfang Tang; Benhai Xiong

doi:10.1016/j.talanta.2023.124401

Au@SiO₂ SERS nanotags based lateral flow immunoassay for simultaneous detection of aflatoxin B₁ and ochratoxin A

Talanta. 2023 Jun 1:258:124401. doi: 10.1016/j.talanta.2023.124401. Epub 2023 Feb 28.

Authors

Ruipeng Chen¹, Hui Wang¹, Chaoqun Sun², Yiguang Zhao¹, Yue He¹, Muhammad Shemyal Nisar³, Wensong Wei⁴, Haiqi Kang⁵, Xiulan Xie¹, Chunmei Du¹, Qingyao Luo¹, Liang Yang⁶, Xiangfang Tang⁷, Benhai Xiong⁸

Affiliations

¹ State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
² College of Animal Science & Technology, Guangxi University, Nanning, Guangxi, 530004, China.
³ Sino-British College, University of Shanghai for Science and Technology, Shanghai, 200093, China.
⁴ Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
⁵ College of Economics and Management, Beijing University of Agriculture, Beijing, 102206, China.
⁶ State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. Electronic address: yangliang@caas.cn.
⁷ State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. Electronic address: tangxiangfang@caas.cn.
⁸ State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. Electronic address: xiongbenhai@caas.cn.

PMID: 36867957
DOI: 10.1016/j.talanta.2023.124401

Abstract

Agricultural products are frequently contaminated by mycotoxins. Multiplex, ultrasensitive, and rapid determination of mycotoxins is still a challenging problem, which is of great significance to food safety and public health. Herein, a surface-enhanced Raman scattering (SERS) based lateral flow immunoassay (LFA) for the simultaneous on-site determination of aflatoxin B₁ (AFB₁) and ochratoxin A (OTA) on the same test line (T line) was developed, in this study. In practice, two kinds of Raman reporters 4-mercaptobenzoic acid (4-MBA), and 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) encoded silica-encapsulated gold nanotags (Au^4-MBA@SiO₂ and Au^DNTB@SiO₂) were used as detection markers to identify the two different mycotoxins. Through systematic optimization of the experimental conditions, this biosensor has high sensitivity and multiplexing with the limits of detection (LODs) at 0.24 pg mL^-1 for AFB₁ and 0.37 pg mL^-1 for OTA. These are far below the regulatory limits set by the European Commission, in which the minimum LODs for AFB₁ and OTA are 2.0 and 3.0 μg kg^-1. In the spiked experiment, the food matrix are corn, rice, and wheat, and the mean recoveries of the two mycotoxins ranged from 91.0% ± 6.3%-104.8% ± 5.6% for AFB₁ and 87.0% ± 4.2%-112.0% ± 3.3% for OTA. These results demonstrate that the developed immunoassay has good stability, selectivity, and reliability, which can be used for routine monitoring of mycotoxin contamination.

Keywords: Au@SiO(2) SERS nanotag; High-sensitive detection; Multiplex mycotoxins detection; SERS-LFA.

MeSH terms

Aflatoxin B1 / analysis
Gold
Immunoassay
Limit of Detection
Metal Nanoparticles*
Mycotoxins* / analysis
Reproducibility of Results
Silicon Dioxide

Substances

ochratoxin A
4-mercaptobutyramidine
Aflatoxin B1
Silicon Dioxide
Mycotoxins
Gold