Product-driven high-throughput screening of industrial filamentous actinomycetes

Yue Zhang; Xuemei Zhang; Meng Wang

doi:10.1016/j.tibtech.2023.02.004

Product-driven high-throughput screening of industrial filamentous actinomycetes

Trends Biotechnol. 2023 Sep;41(9):1109-1112. doi: 10.1016/j.tibtech.2023.02.004. Epub 2023 Feb 28.

Authors

Yue Zhang¹, Xuemei Zhang², Meng Wang³

Affiliations

¹ Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China. Electronic address: zhangy@tib.cas.cn.
² Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China; Haihe Laboratory of Synthetic Biology, Tianjin 300308, China; School of Life Sciences, University of Science and Technology of China, Hefei 230026, China.
³ Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China. Electronic address: wangmeng@tib.cas.cn.

PMID: 36863908
DOI: 10.1016/j.tibtech.2023.02.004

Abstract

Traditional strain breeding of industrial filamentous actinomycetes has long been hampered by insufficient screening throughput. From microtiter plate based methods to droplet-based microfluidic screening, various novel product-driven high-throughput screening (HTS) methods have pushed the screening speed limit towards a minimum of hundreds of strains per second with single cell resolution.

Keywords: filamentous actinomycete; high productivity; high-throughput screening; product-driven; strain engineering.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Actinobacteria*
Actinomyces
Bioreactors
High-Throughput Screening Assays
Microfluidics