Water-soluble single molecular probe for simultaneous detection of viscosity and hydrazine

Yu Jin; Ruitao Sun; Guangqiang Li; Mi Yuan; Weichong Shao; Minhui Cao; Chao Yuan; Suhua Wang

doi:10.1016/j.saa.2023.122558

Water-soluble single molecular probe for simultaneous detection of viscosity and hydrazine

Spectrochim Acta A Mol Biomol Spectrosc. 2023 Jun 5:294:122558. doi: 10.1016/j.saa.2023.122558. Epub 2023 Feb 26.

Authors

Yu Jin¹, Ruitao Sun¹, Guangqiang Li¹, Mi Yuan¹, Weichong Shao¹, Minhui Cao², Chao Yuan³, Suhua Wang⁴

Affiliations

¹ College of Science, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
² College of Science, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: cmhxsz@mail.hzau.edu.cn.
³ College of Science, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Guangdong Provincial Key Laboratory of Petrochemical Pollution Processes and Control, School of Environmental Science and Engineering, Guangdong University of Petrochemical Technology, Maoming 525000, China. Electronic address: yuanchao@gdupt.edu.cn.
⁴ Guangdong Provincial Key Laboratory of Petrochemical Pollution Processes and Control, School of Environmental Science and Engineering, Guangdong University of Petrochemical Technology, Maoming 525000, China. Electronic address: wangsh@gdupt.edu.cn.

PMID: 36863083
DOI: 10.1016/j.saa.2023.122558

Abstract

Hydrazine (N₂H₄) can cause serious damage to human health, while intracellular viscosity is highly associated with many diseases and cellular dysfunctions. Herein, we report the synthesis of a dual-responsive organic molecule-based fluorescent probe with excellent water solubility being capable of detection of N₂H₄ and viscosity through dual-fluorescence channels in "turn on" manner for both. Besides sensitive detection of N₂H₄ in aqueous solution with detection limit of 0.135 μM, this probe could be used for vapor N₂H₄ detection in colorimetric and fluorescent manners. In addition, the probe demonstrated viscosity-dependent fluorescence enhancement behavior, and as high as 150-fold enhancement could be obtained at 95% glycerol aqueous solution. Cell imaging experiment revealed that the probe could be used for the discriminating of living and dead cells.

Keywords: Cell imaging; Dual-plex detection; Fluorescence sensor; Gas detection.

MeSH terms

Fluorescent Dyes
HeLa Cells
Humans
Hydrazines
Molecular Probes*
Spectrometry, Fluorescence / methods
Viscosity
Water*

Substances

Molecular Probes
Water
Fluorescent Dyes
hydrazine
Hydrazines